Background: Esophageal cancer is one of the most common cancer worldwide with poor prognosis and high mortality. SNAI1 transcription factors, coding Snail1, it is important for the development of esophageal cancer metastasis while microRNA (miRNA) -203 has been shown to function as inhibitors of metastasis in the EC. The EC Snail1 protein is stabilized in part by deubiquitinating enzymes USP26; However, how USP26 set is not fully known.
Methods: Expression SNAI1 and USP26 messenger RNA (mRNA) and miR-203 performed on datasets in The Cancer Genome Atlas and Gene Expression Omnibus, respectively. Snail1 and USP26 protein expression and miR-203 are determined in normal esophageal cell lines HET-1A and Kyse150 EC cell lines and TE-1 using western blot and quantitative polymerase chain reaction, respectively. TargetScan used for in situ predicted miR-203 targets and in vitro assays using a heterologous reporter wild-type and miR-203 mutant seeds from the 3 ‘translated region (UTR) of USP26 is used to investigate whether USP26 is a target of miR-203.
The effect of the increase in miR-203 using MIR203A / 5P mimic the USP26 and Snail1 in HET-1A, Kyse150 and TE-1 cell line is done by using western blot analysis of the stability of protein-based and cycloheximide. Modulating effect of miR-203 in Kyse150 and TE-1 cell lines in vitro pro-metastatic effect was analyzed by test invasion, early test wound healing, and chemosensitivity to 5-fluorouracil (5-FU). In vivo lung metastasis assay was used to study the effect of modulation of miR-203 in cells Kyse150.
Targeting deubiquitinating enzyme USP26 by microRNA-203 regulates Snail1’s pro-metastatic functions in esophageal cancer
Results: The mRNA SNAI1 and HSA / MIR203 higher and lower, respectively, in patients with EC compared with tumor-adjacent normal tissue. No changes USP26 mRNA expression was observed in this dataset. MIR / 203 expression was downregulated while the protein expression of both Snail1 and USP26 higher EC Kyse150 cell lines and TE-1 compared to normal esophageal cell lines HET-1A. USP26 is estimated as a potential target of miR-203 by TargetScan Release 2.0. Reporter tests confirmed USP26 as a target of miR-203 in the EC cell line.
Transfection of cell lines with MIR203 imitating EC decreased USP26 protein expression and protein stability Snail1 showed miR-203’s ability to regulate protein levels Snail1 through USP26. exogenous increase in miR-203 in cell lines significantly inhibited Snail EC-1 mediated in vitro function of pro-metastatic invasion, wound healing, and increased chemosensitivity to 5-FU. Finally, overexpression of miR-203 inhibited in vivo lung metastasis Kyse150 cells, which reversed following overexpression of USP26, suggesting a direct role of the regulation of miR-203-mediated metastasis USP26 in the development of EC.
Conclusion: Cumulatively, these results establish an important mechanism whereby a decrease in the expression of miR-203 potentiates the development of metastases in the EC through USP26-mediated stabilization Snail1. Therefore, miR-203 can serve as a biomarker of metastasis in EC and is a potential target for therapeutic intervention in the EC.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Mouse Pancreas PrimaCell2: Normal Pancreatic Islets
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
cDNA from Monkey (Cynomolgus) Normal Tissue: Pancreas
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Frozen Tissue Section - Human Diabetic Tissue: Pancreas
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Adult Normal: Pancreas
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Pancreas Tumor: Adenocarcinoma
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Paraffin Tissue Section - Human Diabetic Tissue: Pancreas
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Phospholipase A2, Pancreas (pPLA2) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Phospholipase A2, Pancreas (pPLA2) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Recombinant Pancreas Specific Transcription Factor 1a (PTF1a)
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein from Human Diabetic Tissue: Pancreas
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Phospholipase A2, Pancreas (pPLA2) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Phospholipase A2, Pancreas (pPLA2) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: Pancreatic cancer tissue array contains 96 cases of pancreatic cancers and 6 cases of normal and non-malignant pancreatic tissues with grading and TNM staging data.
Description: A competitive ELISA for quantitative measurement of Human Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
FFPE Genomic DNA - Human Adult Normal Tissue: Pancreas
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Adult Normal Tissue: Pancreas
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Goat Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Pancreas Specific Transcription Factor 1a ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Pancreas Specific Transcription Factor 1a in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Total RNA from Human Adult Normal Tissue: Pancreas
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Monkey (Rhesus) Normal Tissue: Pancreas
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Total RNA from Monkey (Cynomolgus) Normal Tissue: Pancreas
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Pancreas Specific Transcription Factor 1a (PTF1A) Antibody (HRP)
adverse cardiac fibrosis is involved in cardiac remodeling and heart failure, which is a major cause of heart function deteriorated. accumulative evidence has been explained that microRNAs (miRNAs) play an important role in the pathogenesis of cardiac fibrosis. However, the exact molecular mechanisms underlying the miR-144 in cardiac fibrosis is still unknown. In this study, transverse aortic constriction (TAC) and a rat model of angiotensin II (Ang II) induced cardiac fibroblasts (CF) was created to investigate the expression level of miR-144.
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 Polyclonal Antibody (Human), APC)
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