MicroRNA-184 negatively regulates corneal epithelial wound healing via targeting CDC25A, CARM1, and LASP1

MicroRNA-184 negatively regulates corneal epithelial wound healing via targeting CDC25A, CARM1, and LASP1

Background: MicroRNAs (miRNAs) play an important role in development and homeostasis functional cornea. Our previous research identified miR-184 as one of the most highly expressed miRNAs in the corneal epithelium. Although the expression levels drop dramatically during corneal epithelial wound healing (CEWH), its proper role in mediating the renewal of the corneal epithelium is unresolved. This study aims to reveal the function and mechanism of miR-184 in regulating CEWH.


Methods: Quantitative RT-PCR analysis indicated miR-184 expression patterns during CEWH in mice. Ectopic miR-184 injection is determined effect on this process in vivo. We evaluated the effects of miR-184 and target gene on proliferation, cell cycle and cell migration of human corneal epithelial cells (HCECs) using the MTS, flow cytometry, and the test wound healing, respectively. bioinformatics analysis, in conjunction with microarray gene analysis and cell-based luciferase tests, the target genes of miR-184 pointed to contribute CEWH.


Results: MiR-184 underwent marked downregulation during CEWH mice. Ectopic miR-184 excess delayed this process in mice. Furthermore, miR-184 transfection into HCECs significantly inhibited cell proliferation, cell cycle progression and cell migration. MiR-184 is targeted directly CDC25A, CARM1, and LASP1, and downregulated the expression of them in HCECs. CARM1 HCEC downregulation inhibits both proliferation and migration, while the only decrease migration LASP1 gene expression is inhibited.

Conclusion: Our results suggest that miR-184 inhibit corneal epithelial cell proliferation and migration through targeting CDC25A, CARM1, and LASP1, showing it acts as a negative modulator for CEWH. Therefore, identifying strategies to suppress the expression level of miR-184 has the potential to promote CEWH.

 MicroRNA-184 negatively regulates corneal epithelial wound healing via targeting CDC25A, CARM1, and LASP1
MicroRNA-184 negatively regulates corneal epithelial wound healing via targeting CDC25A, CARM1, and LASP1

Uromodulin and microRNAs in Kidney Transplant-association with Kidney Graft Function

Uromodulin and microRNAs (miRNAs) have recently been investigated as a potential biomarker for kidney transplantation related pathology and outcomes, with a particular focus on biomarkers indicate a specific disease process and the survival of the kidney graft. The purpose of this study was to determine whether serum concentrations uromodulin expression and miRNAs selected may be associated with kidney function in kidney transplant recipients (KTRs).

Concentration uromodulin and expression of six miRNAs were selected (miR-29c, miR-126, miR-146a, miR-150, miR-155 and miR-223) were determined in serum of 100 KTRs with stable function corruption and chronic kidney disease all five Step. Kidney graft function was estimated by routine parameters (creatinine, urea, cystatin C, and the Chronic Kidney Disease Epidemiology Collaboration equation studies) and precisely measured by using chromium-51 labeled ethylenediaminetetraacetic acid permission. miRNAs that have shown to be independent of renal graft function, demonstrate their potential as biomarkers related to renal graft disease process.

Rat Mammary Gland, E16 cDNA

RD-414-16 30 reactions
EUR 243

Rat Mammary Gland, E17 cDNA

RD-414-17 30 reactions
EUR 243

Rat Mammary Gland, E18 cDNA

RD-414-18 30 reactions
EUR 243

Rat Mammary Gland, E19 cDNA

RD-414-19 30 reactions
EUR 243

Rat Mammary Gland, E20 cDNA

RD-414-20 30 reactions
EUR 243

Rat Mammary Gland Western Blot

RW-414 1 Blot
EUR 668

Rat Mammary Gland, E12 Total RNA

RR-414-12 0.05mg
EUR 160

Rat Mammary Gland, E13 Total RNA

RR-414-13 0.05mg
EUR 160

Rat Mammary Gland, E14 Total RNA

RR-414-14 0.05mg
EUR 160

Rat Mammary Gland, E15 Total RNA

RR-414-15 0.05mg
EUR 160

Rat Mammary Gland, E16 Total RNA

RR-414-16 0.05mg
EUR 160

Rat Mammary Gland, E17 Total RNA

RR-414-17 0.05mg
EUR 160

Rat Mammary Gland, E18 Total RNA

RR-414-18 0.05mg
EUR 160

Rat Mammary Gland, E19 Total RNA

RR-414-19 0.05mg
EUR 160

Rat Mammary Gland, E20 Total RNA

RR-414-20 0.05mg
EUR 160

Rat Mammary Gland, E12 Total Protein

RT-414-12 0.5mg
EUR 153

Rat Mammary Gland, E13 Total Protein

RT-414-13 0.5mg
EUR 153

Rat Mammary Gland, E14 Total Protein

RT-414-14 0.5mg
EUR 153

Rat Mammary Gland, E15 Total Protein

RT-414-15 0.5mg
EUR 153

Rat Mammary Gland, E16 Total Protein

RT-414-16 0.5mg
EUR 153

Rat Mammary Gland, E17 Total Protein

RT-414-17 0.5mg
EUR 153

Rat Mammary Gland, E18 Total Protein

RT-414-18 0.5mg
EUR 153

Rat Mammary Gland, E19 Total Protein

RT-414-19 0.5mg
EUR 153

Rat Mammary Gland, E20 Total Protein

RT-414-20 0.5mg
EUR 153

Rat Mammary Gland, non pregnant cDNA

RD-414 30 reactions
EUR 243

Rat Mammary Gland Frozen Sections, E12

RF-414-12 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, E13

RF-414-13 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, E14

RF-414-14 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, E15

RF-414-15 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, E16

RF-414-16 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, E17

RF-414-17 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, E18

RF-414-18 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, E19

RF-414-19 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, E20

RF-414-20 10 slides
EUR 266

Rabbit Mammary Gland cDNA

TD-414 30 reactions
EUR 243

Rat Mammary Gland Premade Northern Blot

RN-414 1 Blot
EUR 668

Rat Mammary Gland Paraffin Sections, E12

RP-414-12 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, E13

RP-414-13 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, E14

RP-414-14 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, E15

RP-414-15 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, E16

RP-414-16 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, E17

RP-414-17 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, E18

RP-414-18 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, E19

RP-414-19 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, E20

RP-414-20 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Virgin

RF-414-V 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Virgin

RP-414-V 10 slides
EUR 266

Rat Mammary Gland Developmental Western Blot

RW-414-D 1 Blot
EUR 910

Rat Mammary Gland, non pregnant Total RNA*

RR-414 0.05mg
EUR 160

Rat Mammary Gland Developmental Northern Blot

RN-414-D 1 Blot
EUR 910

Rat Mammary Gland, non pregnant Total Protein

RT-414 0.5mg
EUR 153

Rabbit Mammary Gland Total RNA

TR-414 0.05mg
EUR 160

Rat Mammary Gland, Day 1 of Lactation cDNA

RD-414-L1 30 reactions
EUR 243

Rat Mammary Gland, Day 3 of Lactation cDNA

RD-414-L3 30 reactions
EUR 243

Rat Mammary Gland, Day 7 of Lactation cDNA

RD-414-L7 30 reactions
EUR 243

Rat Mammary Gland Frozen Sections, Lactation D1

RF-414-L1 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Lactation D3

RF-414-L3 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Lactation D7

RF-414-L7 10 slides
EUR 266

Rat WS Mammary Gland, non pregnant Total RNA*

RR-414-WS 0.05mg
EUR 160

Rat Mammary Gland, Day 1 of Involution cDNA

RD-414-N1 30 reactions
EUR 243

Rat Mammary Gland, Day 7 of Involution cDNA

RD-414-N7 30 reactions
EUR 243

Rat Mammary Gland Frozen Sections, Involution D1

RF-414-N1 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Involution D7

RF-414-N7 10 slides
EUR 266

Rat Mammary Gland Frozen Sections, Non-Pregnant

RF-414 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D1

RP-414-L1 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D3

RP-414-L3 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D7

RP-414-L7 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Involution D1

RP-414-N1 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Involution D7

RP-414-N7 10 slides
EUR 266

Rat Mammary Gland Paraffin Sections, Non-Pregnant

RP-414 10 slides
EUR 266

Rat WS Mammary Gland, non pregnant Total Protein

RT-414-WS 0.5mg
EUR 153

Mouse Mammary Gland Developmental NB

MN-414-D 1 Blot
EUR 910

Mouse CD1 Mammary Gland, E11 cDNA

MD-414-11 30 reactions
EUR 243

Mouse C57 Mammary Gland, E11 cDNA

MD-414-11-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E12 cDNA

MD-414-12 30 reactions
EUR 243

Mouse C57 Mammary Gland, E12 cDNA

MD-414-12-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E13 cDNA

MD-414-13 30 reactions
EUR 243

Mouse C57 Mammary Gland, E13 cDNA

MD-414-13-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E14 cDNA

MD-414-14 30 reactions
EUR 243

Mouse C57 Mammary Gland, E14 cDNA

MD-414-14-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E15 cDNA

MD-414-15 30 reactions
EUR 243

Mouse C57 Mammary Gland, E15 cDNA

MD-414-15-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E16 cDNA

MD-414-16 30 reactions
EUR 243

Mouse C57 Mammary Gland, E16 cDNA

MD-414-16-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E17 cDNA

MD-414-17 30 reactions
EUR 243

Mouse C57 Mammary Gland, E17 cDNA

MD-414-17-C57 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E18 cDNA

MD-414-18 30 reactions
EUR 243

Mouse C57 Mammary Gland, E18 cDNA

MD-414-18-C57 30 reactions
EUR 280

Rat Mammary Gland, Day 1 of Lactation Total RNA

RR-414-L1 0.05mg
EUR 160

Rat Mammary Gland, Day 3 of Lactation Total RNA

RR-414-L3 0.05mg
EUR 160

Rat Mammary Gland, Day 7 of Lactation Total RNA

RR-414-L7 0.05mg
EUR 160

Guinea Pig Mammary Gland-E20 cDNA

GD-414-20 30 reactions
EUR 280

Guinea Pig Mammary Gland-E25 cDNA

GD-414-25 30 reactions
EUR 280

Guinea Pig Mammary Gland-E35 cDNA

GD-414-35 30 reactions
EUR 280

Guinea Pig Mammary Gland-E40 cDNA

GD-414-40 30 reactions
EUR 280

Rat Mammary Gland, non pregnant cDNA-Random Primer

RD-414-RH 30 reactions
EUR 243

Rat Mammary Gland, Day 1 of involution Total RNA

RR-414-N1 0.05mg
EUR 160

Rat Mammary Gland, Day 7 of involution Total RNA

RR-414-N7 0.05mg
EUR 160

Human Breast (Mammary Gland) Total RNA

HR-414 0.05mg
EUR 229

Mouse CD1 Mammary Gland Western Blot

MW-414 1 Blot
EUR 668

Mouse C57 Mammary Gland Western Blot

MW-414-C57 1 Blot
EUR 789

Rat WS Mammary Gland Paraffin Sections, Non-Pregnant

RP-414-WS 10 slides
EUR 266

Rat Mammary Gland, Day 1 of Lactation Total Protein

RT-414-L1 0.5mg
EUR 153

Rat Mammary Gland, Day 3 of Lactation Total Protein

RT-414-L3 0.5mg
EUR 153

Rat Mammary Gland, Day 7 of Lactation Total Protein

RT-414-L7 0.5mg
EUR 153

Rat WS Mammary Gland, non pregnant cDNA-Oligo-dT

RD-414-WS 30 reactions
EUR 243

Rat Mammary Gland, Day 1 of Involution Total Protein

RT-414-N1 0.5mg
EUR 153

Rat Mammary Gland, Day 7 of Involution Total Protein

RT-414-N7 0.5mg
EUR 153

Rat Mammary Gland cDNA Panel, set of any 5 Tissues

RD-414-005 5x10 reactions
EUR 867

Rat Mammary Gland cDNA Panel, set of any 10 Tissues

RD-414-010 10x10 reactions
EUR 1158

Rat Mammary Gland cDNA Panel, set of any 15 Tissues

RD-414-015 15x10 reactions
EUR 1449

Rat mammary gland Frozen Sections Panel, any 8 stages

RF-414-008 8X4 slides
EUR 744

Mouse CD1 Mammary Gland, E11 Total RNA

MR-414-11 0.05mg
EUR 160

Mouse C57 Mammary Gland, E11 Total RNA

MR-414-11-C57 0.025mg
EUR 180

Mouse CD1 Mammary Gland, E12 Total RNA

MR-414-12 0.05mg
EUR 160

Mouse C57 Mammary Gland, E12 Total RNA

MR-414-12-C57 0.025mg
EUR 180

Mouse CD1 Mammary Gland, E13 Total RNA

MR-414-13 0.05mg
EUR 160

Mouse C57 Mammary Gland, E13 Total RNA

MR-414-13-C57 0.025mg
EUR 180

Mouse CD1 Mammary Gland, E14 Total RNA

MR-414-14 0.05mg
EUR 160

Mouse C57 Mammary Gland, E14 Total RNA

MR-414-14-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, E15 Total RNA

MR-414-15 0.05mg
EUR 160

Mouse C57 Mammary Gland, E15 Total RNA

MR-414-15-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, E16 Total RNA

MR-414-16 0.05mg
EUR 160

Mouse C57 Mammary Gland, E16 Total RNA

MR-414-16-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, E17 Total RNA

MR-414-17 0.05mg
EUR 160

Mouse C57 Mammary Gland, E17 Total RNA

MR-414-17-C57 0.05mg
EUR 180

Mouse CD1 Mammary Gland, E18 Total RNA

MR-414-18 0.05mg
EUR 160

Mouse C57 Mammary Gland, E18 Total RNA

MR-414-18-C57 0.05mg
EUR 180

Rat mammary gland Frozen Sections Panel, any 16 tissues

RF-414-016 16X4 slides
EUR 1035

Human Breast (Mammary Gland) Frozen Sections

HF-414 10Slides
EUR 218

Rat mammary gland Paraffin Sections Panel, any 8 stages

RP-414-008 8X4 slides
EUR 744

Rat mammary gland Paraffin Sections Panel, any 16 stages

RP-414-016 16X4 slides
EUR 1035

Human Breast (Mammary Gland) Paraffin Section

HP-414 10 slides
EUR 228

Guinea pig Mammary Gland-E20 Total RNA

GR-414-20 0.1mg
EUR 192

Guinea pig Mammary Gland-E25 Total RNA

GR-414-25 0.1mg
EUR 192

Guinea pig Mammary Gland-E35 Total RNA

GR-414-35 0.1mg
EUR 192

Guinea pig Mammary Gland-E40 Total RNA

GR-414-40 0.1mg
EUR 192

Mouse CD1 Mammary Gland, non pregnant cDNA

MD-414 30 reactions
EUR 243

Mouse CD1 Mammary Gland, non pregnant cDNA

MD-414-1 30 reactions
EUR 243

Mouse BLC Mammary Gland, non pregnant cDNA

MD-414-BLC 30 reactions
EUR 280

Mouse C57 Mammary Gland, non pregnant cDNA

MD-414-C57 30 reactions
EUR 280

Mouse C57 Mammary Gland, non pregnant cDNA

MD-414-C57-1 30 reactions
EUR 280

Mouse CD1 Mammary Gland, E11 Total Protein

MT-414-11 0.5mg
EUR 153

Mouse C57 Mammary Gland, E11 Total Protein

MT-414-11-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E12 Total Protein

MT-414-12 0.5mg
EUR 153

Mouse C57 Mammary Gland, E12 Total Protein

MT-414-12-C57 0.5mg
EUR 180

Mouse CD1 Mammary Gland, E13 Total Protein

MT-414-13 0.5mg
EUR 153

In contrast, serum uromodulin level depends entirely on kidney graft function and thus reflected tubular function rather than a specific injury kidney transplant. However, a decrease in the concentration of serum uromodulin can be observed in the initial trip tubulointerstitial injury, thus indicating a useful role as an accurate, noninvasive biomarker of early (subclinical) renal graft injury.

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