Background: MicroRNAs (miRNAs) play an important role in development and homeostasis functional cornea. Our previous research identified miR-184 as one of the most highly expressed miRNAs in the corneal epithelium. Although the expression levels drop dramatically during corneal epithelial wound healing (CEWH), its proper role in mediating the renewal of the corneal epithelium is unresolved. This study aims to reveal the function and mechanism of miR-184 in regulating CEWH.
Methods: Quantitative RT-PCR analysis indicated miR-184 expression patterns during CEWH in mice. Ectopic miR-184 injection is determined effect on this process in vivo. We evaluated the effects of miR-184 and target gene on proliferation, cell cycle and cell migration of human corneal epithelial cells (HCECs) using the MTS, flow cytometry, and the test wound healing, respectively. bioinformatics analysis, in conjunction with microarray gene analysis and cell-based luciferase tests, the target genes of miR-184 pointed to contribute CEWH.
Results: MiR-184 underwent marked downregulation during CEWH mice. Ectopic miR-184 excess delayed this process in mice. Furthermore, miR-184 transfection into HCECs significantly inhibited cell proliferation, cell cycle progression and cell migration. MiR-184 is targeted directly CDC25A, CARM1, and LASP1, and downregulated the expression of them in HCECs. CARM1 HCEC downregulation inhibits both proliferation and migration, while the only decrease migration LASP1 gene expression is inhibited.
Conclusion: Our results suggest that miR-184 inhibit corneal epithelial cell proliferation and migration through targeting CDC25A, CARM1, and LASP1, showing it acts as a negative modulator for CEWH. Therefore, identifying strategies to suppress the expression level of miR-184 has the potential to promote CEWH.
MicroRNA-184 negatively regulates corneal epithelial wound healing via targeting CDC25A, CARM1, and LASP1
Uromodulin and microRNAs in Kidney Transplant-association with Kidney Graft Function
Uromodulin and microRNAs (miRNAs) have recently been investigated as a potential biomarker for kidney transplantation related pathology and outcomes, with a particular focus on biomarkers indicate a specific disease process and the survival of the kidney graft. The purpose of this study was to determine whether serum concentrations uromodulin expression and miRNAs selected may be associated with kidney function in kidney transplant recipients (KTRs).
Concentration uromodulin and expression of six miRNAs were selected (miR-29c, miR-126, miR-146a, miR-150, miR-155 and miR-223) were determined in serum of 100 KTRs with stable function corruption and chronic kidney disease all five Step. Kidney graft function was estimated by routine parameters (creatinine, urea, cystatin C, and the Chronic Kidney Disease Epidemiology Collaboration equation studies) and precisely measured by using chromium-51 labeled ethylenediaminetetraacetic acid permission. miRNAs that have shown to be independent of renal graft function, demonstrate their potential as biomarkers related to renal graft disease process.
Description: A sandwich ELISA for quantitative measurement of Rat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Human salivary gland tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human salivary gland tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the salivary gland tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The salivary gland tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human salivary gland tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human salivary gland tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated salivary gland tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated salivary gland tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Normal pituitary gland tissue array, 25 cases. In most of the cases, two cores were taken, one from anterior lobe and the other from posterior lobe.
Description: A competitive ELISA for quantitative measurement of Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Endocrine gland vascular endothelial growth factor, EG- VEGF
Description: A sandwich ELISA for quantitative measurement of Rat Endocrine gland vascular endothelial growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Endocrine gland vascular endothelial growth factor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Endocrine gland vascular endothelial growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Endocrine gland vascular endothelial growth factor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Endocrine gland vascular endothelial growth factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Pituitary gland adenoma tissue array, 45 cases including a single core from 42 cases of various types of adenoma and two cores (one from anterior lobe and the other from posterior lobe) from each of three control normal pituitary glands.
Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells Growth Medium
Description: Quantitativesandwich ELISA kit for measuring Mouse mammary carcinoma Marker-CA153 in samples from serum, plasma, homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse mammary carcinoma Marker-CA153 in samples from serum, plasma, homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Mammary Serine Protease Inhibitor (Maspin) in serum, plasma, tissue homogenates and other biological fluids.
Human Mammary Serine Protease Inhibitor (Maspin) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Mammary Serine Protease Inhibitor (Maspin) in serum, plasma, tissue homogenates and other biological fluids.
Human Mammary Serine Protease Inhibitor (Maspin) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Mammary Serine Protease Inhibitor (Maspin) in serum, plasma, tissue homogenates and other biological fluids.
Human Mammary Serine Protease Inhibitor (Maspin) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Mammary Serine Protease Inhibitor (Maspin) in serum, plasma, tissue homogenates and other biological fluids.
Human Mammary Serine Protease Inhibitor (Maspin) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Mammary Serine Protease Inhibitor (Maspin) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Human mammary derived growth inhibitor(MDGI)ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Mammary Serine Protease Inhibitor (Maspin) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Mammary Serine Protease Inhibitor (Maspin) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Mammary Serine Protease Inhibitor (Maspin) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human mammary derived growth inhibitor,MDGI ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Rat Endocrine gland vascular endothelial growth factor, EG-VEGF in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat Endocrine gland vascular endothelial growth factor, EG-VEGF ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Rat Endocrine gland vascular endothelial growth factor, EG-VEGF in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Rat Endocrine gland vascular endothelial growth factor(EG-VEGF)ELISA Kit
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Frozen Tissue Section - Human Adult Normal: Submaxillary Gland
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
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In contrast, serum uromodulin level depends entirely on kidney graft function and thus reflected tubular function rather than a specific injury kidney transplant. However, a decrease in the concentration of serum uromodulin can be observed in the initial trip tubulointerstitial injury, thus indicating a useful role as an accurate, noninvasive biomarker of early (subclinical) renal graft injury.
Douglas
