Background: MicroRNAs (miRNAs) play an important role in development and homeostasis functional cornea. Our previous research identified miR-184 as one of the most highly expressed miRNAs in the corneal epithelium. Although the expression levels drop dramatically during corneal epithelial wound healing (CEWH), its proper role in mediating the renewal of the corneal epithelium is unresolved. This study aims to reveal the function and mechanism of miR-184 in regulating CEWH.

Methods: Quantitative RT-PCR analysis indicated miR-184 expression patterns during CEWH in mice. Ectopic miR-184 injection is determined effect on this process in vivo. We evaluated the effects of miR-184 and target gene on proliferation, cell cycle and cell migration of human corneal epithelial cells (HCECs) using the MTS, flow cytometry, and the test wound healing, respectively. bioinformatics analysis, in conjunction with microarray gene analysis and cell-based luciferase tests, the target genes of miR-184 pointed to contribute CEWH.

Results: MiR-184 underwent marked downregulation during CEWH mice. Ectopic miR-184 excess delayed this process in mice. Furthermore, miR-184 transfection into HCECs significantly inhibited cell proliferation, cell cycle progression and cell migration. MiR-184 is targeted directly CDC25A, CARM1, and LASP1, and downregulated the expression of them in HCECs. CARM1 HCEC downregulation inhibits both proliferation and migration, while the only decrease migration LASP1 gene expression is inhibited.

Conclusion: Our results suggest that miR-184 inhibit corneal epithelial cell proliferation and migration through targeting CDC25A, CARM1, and LASP1, showing it acts as a negative modulator for CEWH. Therefore, identifying strategies to suppress the expression level of miR-184 has the potential to promote CEWH.

Uromodulin and microRNAs in Kidney Transplant-association with Kidney Graft Function

Uromodulin and microRNAs (miRNAs) have recently been investigated as a potential biomarker for kidney transplantation related pathology and outcomes, with a particular focus on biomarkers indicate a specific disease process and the survival of the kidney graft. The purpose of this study was to determine whether serum concentrations uromodulin expression and miRNAs selected may be associated with kidney function in kidney transplant recipients (KTRs).

Concentration uromodulin and expression of six miRNAs were selected (miR-29c, miR-126, miR-146a, miR-150, miR-155 and miR-223) were determined in serum of 100 KTRs with stable function corruption and chronic kidney disease all five Step. Kidney graft function was estimated by routine parameters (creatinine, urea, cystatin C, and the Chronic Kidney Disease Epidemiology Collaboration equation studies) and precisely measured by using chromium-51 labeled ethylenediaminetetraacetic acid permission. miRNAs that have shown to be independent of renal graft function, demonstrate their potential as biomarkers related to renal graft disease process.

Rat Mammary Gland, E16 cDNA
RD-414-16	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E17 cDNA
RD-414-17	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E18 cDNA
RD-414-18	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E19 cDNA
RD-414-19	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E20 cDNA
RD-414-20	Zyagen	30 reactions	EUR 243

Rat Mammary Gland Western Blot
RW-414	Zyagen	1 Blot	EUR 668

Rat Mammary Gland, E12 Total RNA
RR-414-12	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E13 Total RNA
RR-414-13	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E14 Total RNA
RR-414-14	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E15 Total RNA
RR-414-15	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E16 Total RNA
RR-414-16	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E17 Total RNA
RR-414-17	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E18 Total RNA
RR-414-18	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E19 Total RNA
RR-414-19	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E20 Total RNA
RR-414-20	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, E12 Total Protein
RT-414-12	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, E13 Total Protein
RT-414-13	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, E14 Total Protein
RT-414-14	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, E15 Total Protein
RT-414-15	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, E16 Total Protein
RT-414-16	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, E17 Total Protein
RT-414-17	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, E18 Total Protein
RT-414-18	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, E19 Total Protein
RT-414-19	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, E20 Total Protein
RT-414-20	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, non pregnant cDNA
RD-414	Zyagen	30 reactions	EUR 243

Rat Mammary Gland Frozen Sections, E12
RF-414-12	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, E13
RF-414-13	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, E14
RF-414-14	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, E15
RF-414-15	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, E16
RF-414-16	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, E17
RF-414-17	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, E18
RF-414-18	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, E19
RF-414-19	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, E20
RF-414-20	Zyagen	10 slides	EUR 266

Rat Mammary Gland Premade Northern Blot
RN-414	Zyagen	1 Blot	EUR 668

Rat Mammary Gland Paraffin Sections, E12
RP-414-12	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, E13
RP-414-13	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, E14
RP-414-14	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, E15
RP-414-15	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, E16
RP-414-16	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, E17
RP-414-17	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, E18
RP-414-18	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, E19
RP-414-19	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, E20
RP-414-20	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, Virgin
RF-414-V	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, Virgin
RP-414-V	Zyagen	10 slides	EUR 266

Rat Mammary Gland Developmental Western Blot
RW-414-D	Zyagen	1 Blot	EUR 910

Rat Mammary Gland Developmental Northern Blot
RN-414-D	Zyagen	1 Blot	EUR 910

Rat Mammary Gland, non pregnant Total RNA*
RR-414	Zyagen	0.05mg	EUR 160

Rabbit Mammary Gland cDNA
TD-414	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, non pregnant Total Protein
RT-414	Zyagen	0.5mg	EUR 153

Rat Mammary Gland Frozen Sections, Lactation D1
RF-414-L1	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, Lactation D3
RF-414-L3	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, Lactation D7
RF-414-L7	Zyagen	10 slides	EUR 266

Rat Mammary Gland, Day 1 of Lactation cDNA
RD-414-L1	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, Day 3 of Lactation cDNA
RD-414-L3	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, Day 7 of Lactation cDNA
RD-414-L7	Zyagen	30 reactions	EUR 243

Rat Mammary Gland Frozen Sections, Involution D1
RF-414-N1	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, Involution D7
RF-414-N7	Zyagen	10 slides	EUR 266

Rat WS Mammary Gland, non pregnant Total RNA*
RR-414-WS	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, Day 1 of Involution cDNA
RD-414-N1	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, Day 7 of Involution cDNA
RD-414-N7	Zyagen	30 reactions	EUR 243

Rat Mammary Gland Paraffin Sections, Lactation D1
RP-414-L1	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D3
RP-414-L3	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, Lactation D7
RP-414-L7	Zyagen	10 slides	EUR 266

Rat Mammary Gland Frozen Sections, Non-Pregnant
RF-414	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, Involution D1
RP-414-N1	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, Involution D7
RP-414-N7	Zyagen	10 slides	EUR 266

Rat Mammary Gland Paraffin Sections, Non-Pregnant
RP-414	Zyagen	10 slides	EUR 266

Rabbit Mammary Gland Total RNA
TR-414	Zyagen	0.05mg	EUR 160

Rat WS Mammary Gland, non pregnant Total Protein
RT-414-WS	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, non pregnant cDNA-Random Primer
RD-414-RH	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, Day 1 of Lactation Total RNA
RR-414-L1	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, Day 3 of Lactation Total RNA
RR-414-L3	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, Day 7 of Lactation Total RNA
RR-414-L7	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, Day 1 of involution Total RNA
RR-414-N1	Zyagen	0.05mg	EUR 160

Rat Mammary Gland, Day 7 of involution Total RNA
RR-414-N7	Zyagen	0.05mg	EUR 160

Rat WS Mammary Gland Paraffin Sections, Non-Pregnant
RP-414-WS	Zyagen	10 slides	EUR 266

Mouse Mammary Gland Developmental NB
MN-414-D	Zyagen	1 Blot	EUR 910

Mouse CD1 Mammary Gland, E11 cDNA
MD-414-11	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E11 cDNA
MD-414-11-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E12 cDNA
MD-414-12	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E12 cDNA
MD-414-12-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E13 cDNA
MD-414-13	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E13 cDNA
MD-414-13-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E14 cDNA
MD-414-14	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E14 cDNA
MD-414-14-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E15 cDNA
MD-414-15	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E15 cDNA
MD-414-15-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E16 cDNA
MD-414-16	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E16 cDNA
MD-414-16-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E17 cDNA
MD-414-17	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E17 cDNA
MD-414-17-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E18 cDNA
MD-414-18	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E18 cDNA
MD-414-18-C57	Zyagen	30 reactions	EUR 280

Rat Mammary Gland, Day 1 of Lactation Total Protein
RT-414-L1	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, Day 3 of Lactation Total Protein
RT-414-L3	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, Day 7 of Lactation Total Protein
RT-414-L7	Zyagen	0.5mg	EUR 153

Rat WS Mammary Gland, non pregnant cDNA-Oligo-dT
RD-414-WS	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, Day 1 of Involution Total Protein
RT-414-N1	Zyagen	0.5mg	EUR 153

Rat Mammary Gland, Day 7 of Involution Total Protein
RT-414-N7	Zyagen	0.5mg	EUR 153

Rat mammary gland Frozen Sections Panel, any 8 stages
RF-414-008	Zyagen	8X4 slides	EUR 744

Rat Mammary Gland cDNA Panel, set of any 5 Tissues
RD-414-005	Zyagen	5x10 reactions	EUR 867

Rat Mammary Gland cDNA Panel, set of any 10 Tissues
RD-414-010	Zyagen	10x10 reactions	EUR 1158

Rat Mammary Gland cDNA Panel, set of any 15 Tissues
RD-414-015	Zyagen	15x10 reactions	EUR 1449

Guinea Pig Mammary Gland-E20 cDNA
GD-414-20	Zyagen	30 reactions	EUR 280

Guinea Pig Mammary Gland-E25 cDNA
GD-414-25	Zyagen	30 reactions	EUR 280

Guinea Pig Mammary Gland-E35 cDNA
GD-414-35	Zyagen	30 reactions	EUR 280

Guinea Pig Mammary Gland-E40 cDNA
GD-414-40	Zyagen	30 reactions	EUR 280

Rat mammary gland Frozen Sections Panel, any 16 tissues
RF-414-016	Zyagen	16X4 slides	EUR 1035

Human Breast (Mammary Gland) Total RNA
HR-414	Zyagen	0.05mg	EUR 229

Mouse CD1 Mammary Gland Western Blot
MW-414	Zyagen	1 Blot	EUR 668

Mouse C57 Mammary Gland Western Blot
MW-414-C57	Zyagen	1 Blot	EUR 789

Rat mammary gland Paraffin Sections Panel, any 8 stages
RP-414-008	Zyagen	8X4 slides	EUR 744

Rat mammary gland Paraffin Sections Panel, any 16 stages
RP-414-016	Zyagen	16X4 slides	EUR 1035

Mouse CD1 Mammary Gland, E11 Total RNA
MR-414-11	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E11 Total RNA
MR-414-11-C57	Zyagen	0.025mg	EUR 180

Mouse CD1 Mammary Gland, E12 Total RNA
MR-414-12	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E12 Total RNA
MR-414-12-C57	Zyagen	0.025mg	EUR 180

Mouse CD1 Mammary Gland, E13 Total RNA
MR-414-13	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E13 Total RNA
MR-414-13-C57	Zyagen	0.025mg	EUR 180

Mouse CD1 Mammary Gland, E14 Total RNA
MR-414-14	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E14 Total RNA
MR-414-14-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, E15 Total RNA
MR-414-15	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E15 Total RNA
MR-414-15-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, E16 Total RNA
MR-414-16	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E16 Total RNA
MR-414-16-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, E17 Total RNA
MR-414-17	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E17 Total RNA
MR-414-17-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, E18 Total RNA
MR-414-18	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E18 Total RNA
MR-414-18-C57	Zyagen	0.05mg	EUR 180

Human Breast (Mammary Gland) Frozen Sections
HF-414	Zyagen	10Slides	EUR 218

Human Breast (Mammary Gland) Paraffin Section
HP-414	Zyagen	10 slides	EUR 228

Rat Mammary Gland Total RNA Panel, set of any 5 Tissues
RR-414-005	Zyagen	5X0.025mg	EUR 776

Rat Mammary Gland Total RNA Panel, set of any 10 Tissues
RR-414-010	Zyagen	10x0.025mg	EUR 1067

Rat Mammary Gland Total RNA Panel, set of any 15 Tissues
RR-414-015	Zyagen	15X0.025mg	EUR 1359

Guinea pig Mammary Gland-E20 Total RNA
GR-414-20	Zyagen	0.1mg	EUR 192

Guinea pig Mammary Gland-E25 Total RNA
GR-414-25	Zyagen	0.1mg	EUR 192

Guinea pig Mammary Gland-E35 Total RNA
GR-414-35	Zyagen	0.1mg	EUR 192

Guinea pig Mammary Gland-E40 Total RNA
GR-414-40	Zyagen	0.1mg	EUR 192

Mouse Mammary Gland Developmental Western Blot
MW-414-D	Zyagen	1 Blot	EUR 910

Mouse CD1 Mammary Gland, E11 Total Protein
MT-414-11	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E11 Total Protein
MT-414-11-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E12 Total Protein
MT-414-12	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E12 Total Protein
MT-414-12-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E13 Total Protein
MT-414-13	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E13 Total Protein
MT-414-13-C57	Zyagen	0.5mg	EUR 180

In contrast, serum uromodulin level depends entirely on kidney graft function and thus reflected tubular function rather than a specific injury kidney transplant. However, a decrease in the concentration of serum uromodulin can be observed in the initial trip tubulointerstitial injury, thus indicating a useful role as an accurate, noninvasive biomarker of early (subclinical) renal graft injury.