Background: MicroRNAs (miRNAs) play an important role in development and homeostasis functional cornea. Our previous research identified miR-184 as one of the most highly expressed miRNAs in the corneal epithelium. Although the expression levels drop dramatically during corneal epithelial wound healing (CEWH), its proper role in mediating the renewal of the corneal epithelium is unresolved. This study aims to reveal the function and mechanism of miR-184 in regulating CEWH.
Methods: Quantitative RT-PCR analysis indicated miR-184 expression patterns during CEWH in mice. Ectopic miR-184 injection is determined effect on this process in vivo. We evaluated the effects of miR-184 and target gene on proliferation, cell cycle and cell migration of human corneal epithelial cells (HCECs) using the MTS, flow cytometry, and the test wound healing, respectively. bioinformatics analysis, in conjunction with microarray gene analysis and cell-based luciferase tests, the target genes of miR-184 pointed to contribute CEWH.
Results: MiR-184 underwent marked downregulation during CEWH mice. Ectopic miR-184 excess delayed this process in mice. Furthermore, miR-184 transfection into HCECs significantly inhibited cell proliferation, cell cycle progression and cell migration. MiR-184 is targeted directly CDC25A, CARM1, and LASP1, and downregulated the expression of them in HCECs. CARM1 HCEC downregulation inhibits both proliferation and migration, while the only decrease migration LASP1 gene expression is inhibited.
Conclusion: Our results suggest that miR-184 inhibit corneal epithelial cell proliferation and migration through targeting CDC25A, CARM1, and LASP1, showing it acts as a negative modulator for CEWH. Therefore, identifying strategies to suppress the expression level of miR-184 has the potential to promote CEWH.
MicroRNA-184 negatively regulates corneal epithelial wound healing via targeting CDC25A, CARM1, and LASP1
Uromodulin and microRNAs in Kidney Transplant-association with Kidney Graft Function
Uromodulin and microRNAs (miRNAs) have recently been investigated as a potential biomarker for kidney transplantation related pathology and outcomes, with a particular focus on biomarkers indicate a specific disease process and the survival of the kidney graft. The purpose of this study was to determine whether serum concentrations uromodulin expression and miRNAs selected may be associated with kidney function in kidney transplant recipients (KTRs).
Concentration uromodulin and expression of six miRNAs were selected (miR-29c, miR-126, miR-146a, miR-150, miR-155 and miR-223) were determined in serum of 100 KTRs with stable function corruption and chronic kidney disease all five Step. Kidney graft function was estimated by routine parameters (creatinine, urea, cystatin C, and the Chronic Kidney Disease Epidemiology Collaboration equation studies) and precisely measured by using chromium-51 labeled ethylenediaminetetraacetic acid permission. miRNAs that have shown to be independent of renal graft function, demonstrate their potential as biomarkers related to renal graft disease process.
Description: A sandwich ELISA for quantitative measurement of Rat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Canine Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells
Description: A sandwich ELISA for quantitative measurement of Mouse Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Mammary carcinoma Marker/Carbohydrate antigen15 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Guinea pig Mammary carcinoma Marker in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Quantitativesandwich ELISA kit for measuring Mouse mammary carcinoma Marker-CA153 in samples from serum, plasma, homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse mammary carcinoma Marker-CA153 in samples from serum, plasma, homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
In contrast, serum uromodulin level depends entirely on kidney graft function and thus reflected tubular function rather than a specific injury kidney transplant. However, a decrease in the concentration of serum uromodulin can be observed in the initial trip tubulointerstitial injury, thus indicating a useful role as an accurate, noninvasive biomarker of early (subclinical) renal graft injury.
Douglas
