Posted in Antibodies, Assay Kits, Biology Cells, cDNA, Culture Cells, Exosomes, Isotypes, NATtrol, Panel, Reagents

Identification of a miRNA based model to detect prognostic subgroups in patients with aggressive B-cell lymphoma

To distinguish prognostic subgroups patients with aggressive B cell lymphoma, we analyzed 800 MIRNAS’s expression with Nanostring NCounter Micna Mirna Test on cohort 228 FFPE samples of patients registered in the Ricover-60 trial and megachoep. We identify significant Mirna signatures for the overall survival (OS) (OS) and progressive free survival (PFS) with linear cox regression punished by Laso. The level of high expression of the MIR-130A-3P and MIR-423-5P shows a better prognosis, while the high level of MIR-374B-5P, MIR-590-5P, MIR-186-5P, and MIR-106B-5P-5P increases Patients’ risk level for OS.

Regarding the PFS high expression MIR-365A-5P In addition to two other MIRNAS increases the prognosis and high level of MIR374A-5P, MIR-106B-5P, and MIR-590-5P, connected with an increased risk and poor prognosis. We identified the signature of Mirna to divide patients into two different risk groups. This prognostic model can be used in risk stratification in clinical trials in the future and helps make personalized therapeutic decisions.

Comparative analysis of transcript profile, proteome, and mirna cupffer cells and monocytes

The macrophage population in most mammalian organs consists of different origin cells. The macrophage of the population comes from the erythromiseloid precursor of the wall of the egg yolk bag; Maintenance of the amount of macrophages in the postnatal ontogenesis is practically independent of the hematopoiesis bone marrow. The largest population of the population of embryonic population is found in the central nervous system (Mikroglia) and the liver (Cell Cupffer). Conversely, dermis and mucous membranes become largely colonized by monocytes of bone marrow derivatives which show clear and phenotical plasticity.

In this study, we compare cupffer cells and monocytes using immunofenotype, gene expression profiles, proteome, and microrna collections. The observed difference does not consider the macrophage of the residents’ hearts as pure m2 macrophages or the situation owned by a monocyte has a pure M1 feature. Monocytes show signs of high plasticity and sensitivity to the pattern of molecules related to pathogens (for example, high transcription rates for <i> TLR 2 </ i>, <i> 4 </ i>, <i> 7 </ i>, and <i> 8 </ i>). Conversely, the macrophage of the Heart of the population is clearly involved in the regulations of certain organ functions (nitrogen metabolism, a complementary system protein synthesis).

PMDNE: Prediction of the Mirna-Disease Association based on network embedding and network similarity analysis

Prediction of success from the Mirna-Disease Association is nontrivial for the diagnosis and prognosis of genetic diseases. There are many methods for predicting mirna and disease, but many biological data and complex, and they are often in network form. Ways to accurately use MIRNA features and biological tissue related to diseases to predict unknown associations always become a challenge. Here, we propose PMDNE, a method based on network embedding and network similarity analysis, to predict Mirna’s associations.

At PMDNE, the structure of bipartite graphics network is improved, and the generator runs randomly designed. For embedded vectors, 128 dimensions are used, and predictive accuracy increases significantly. Compared to other network embedding methods, PMDNE is comparable and competitive with the method of art conditions. The method we can solve features extraction features, reduce the dimensions of features, and increase the efficiency of predictions of mirna-disease associations. This method can also be extended to other areas for biomedical tissue predictions.

Effect of lifestyle factors on mirna expression and signal paths: reviews

The term ‘lifestyle’ includes various factors that contribute to maintenance of good health status. Increased evidence shows that lifestyle factors can affect epigenetic mechanisms, such as MIRNAS’s expression. MIRNA dysregulation can modify gene expression and molecular pathways that can cause functional changes. This review summarizes human studies that highlights that diet, physical activity, smoking consumption and alcohol can affect the Mirna engine and some biological functions.

Most Mirnas are involved in molecular lines that affect inflammation, cell cycle regulations and carcinogenesis produce onset or the development of pathological conditions. Investigating this interaction will be very important to understand the etiology of the pathological process, the potential for new care strategies and to prevent disease.

From the Mirna target network to the Mirna function: MIR-375 can regulate apoptosis and dynamics of actin in the heart muscle through the path that depends on RHO-GTPASES

Micrornas (MIRNAS) is a short ribonucleic acid molecule (RNA), without code, which is involved in the main biological process regulations, such as apoptosis or proliferation and cell differentiation, through specific interactions of sequences with the MRNAS target. In this study, we propose workflows to predict the MIRNA function by analyzing their target gene network structure. This workflow is applied to study the functional role of the MIR-375 in the heart of the muscle (myocardium), because Mirna previously proved to be associated with heart disease, and the data on its functions in myocardium was largely unclear.

Hygromycin B
40810000-2 100 mg
EUR 35.69

Hygromycin B
40810000-3 250 mg
EUR 53.99

Hygromycin B
40810000-4 1 g
EUR 67.81

Hygromycin B
40810000-5 5 g
EUR 255.31

Hygromycin B
40810000-8 25 g
EUR 905.02

Hygromycin B
40810000-9 10 g
EUR 493.23

Hygromycin B
abx184120-1g 1 g
EUR 360

Hygromycin B
AT190 1mg
EUR 1336.8

Hygromycin B
A2515-200 200 mg
EUR 40
Description: Suitable for mammalian cell selection

Hygromycin B
A2515-5.1 10 mM (in 1mL DMSO)
EUR 129.6
Description: Hygromycin B is a selective antibiotic.Antibiotics are a type of antimicrobial used in the treatment and prevention of bacterial infection.Hygromycin B is an antibiotic that is effective on most bacteria, fungi and higher eukaryotes.

Hygromycin B
A2515-500 500 mg
EUR 64
Description: Suitable for mammalian cell selection

Hygromycin B
A2813-100MG 100MG
EUR 39.6
Description: Cell Cuture

Hygromycin B
AG190 1 mg
EUR 627.6

Hygromycin B
GA7834 100mg
EUR 347.58

Hygromycin B
G265 1.0 g
EUR 350
Description: Hygromycin B is an aminoglycoside produced by Streptomyces hygroscopicus bacteria. This antibiotic kills fungi, bacteria, and even eukaryotic cells by blocking protein synthesis. Hygromycin B is often used in order to select for and sustain eukaryotic and prokaryotic cells that contain the hygromycin resistance gene.

Hygromycin B
HY-B0490 10mM/1mL
EUR 151.2

Hygromycin B
GA7834-1 1
EUR 95

Hygromycin B
GA7834-100 100
EUR 31.7

Hygromycin B
GA7834-100MG 100 mg
EUR 74.4

Hygromycin B
GA7834-1G 1 g
EUR 151.2

Hygromycin B
GA7834-5 5
EUR 379.6

Hygromycin B
GA7834-500 500
EUR 55.4

Hygromycin B
GA7834-500MG 500 mg
EUR 103.2

Hygromycin B
GA7834-5G 5 g
EUR 495.6

Hygromycin B
H007-1G 1 g
EUR 61.03
Description: C20H37N3O13

Hygromycin B
H007-500MG 500 mg
EUR 33.38
Description: C20H37N3O13

Hygromycin B
H007-5x1G 5 x 1 g
EUR 251.2
Description: C20H37N3O13

Hygromycin B
H997600 100mg
EUR 1759
Description: 31282-04-9

Hygromycin B
T1022-10mg 10mg Ask for price
Description: Hygromycin B

Hygromycin B
T1022-1g 1g Ask for price
Description: Hygromycin B

Hygromycin B
T1022-1mg 1mg Ask for price
Description: Hygromycin B

Hygromycin B
T1022-50mg 50mg Ask for price
Description: Hygromycin B

Hygromycin B
T1022-5mg 5mg Ask for price
Description: Hygromycin B

Hygromycin B
abx082491-100l 100 µl
EUR 337.5

Hygromycin B
abx082491-1ml 1 ml Ask for price

Hygromycin B
abx082491-200l 200 µl Ask for price

Hygromycin B
MBS384891-100mg 100mg
EUR 130

Hygromycin B
MBS384891-1mLinDMSO 1mL(inDMSO)
EUR 145

Hygromycin B
MBS384891-5x1mLinDMSO 5x1mL(inDMSO)
EUR 635

Hygromycin B
MBS340813-10mg 10mg
EUR 4030

Hygromycin B
MBS340813-1mg 1mg
EUR 800

Hygromycin B
MBS340813-5x10mg 5x10mg
EUR 17955

Hygromycin B
MBS342245-05mL 0.5mL
EUR 430

Hygromycin B
MBS342245-5x05mL 5x0.5mL
EUR 1760

Hygromycin B
MBS3604001-100mg 100mg
EUR 200

Hygromycin B
MBS3604001-200mg 200mg
EUR 210

Hygromycin B
MBS3604001-5x200mg 5x200mg
EUR 630

Hygromycin B
MBS575868-1g 1g
EUR 175

Hygromycin B
MBS575868-200mg 200mg
EUR 145

Hygromycin B
MBS575868-500mg 500mg
EUR 155

Hygromycin B
MBS575868-5x1g 5x1g
EUR 645

Hygromycin B, 1g
R3128-1g each
EUR 362.4

Hygromycin B [BSA]
DAG4469 0.5mg
EUR 936

Hygromycin B [KLH]
DAG4485 0.5mg
EUR 1248

Hygromycin B, 10g
R3128 10g
EUR 1918.7

Hygromycin B, 10g
R3128-10g each
EUR 2724

Hygromycin B (HRP)
abx284211-100g 100 µg Ask for price

Hygromycin B (HRP)
abx284211-20g 20 µg
EUR 475

Hygromycin B (HRP)
abx284211-50g 50 µg Ask for price

Hygromycin B (Powder)
MBS651253-100KUnits 100KUnits
EUR 190

Hygromycin B (Powder)
MBS651253-1MUnits 1MUnits
EUR 450

Hygromycin B (Powder)
MBS651253-500KUnits 500KUnits
EUR 310

Hygromycin B (Powder)
MBS651253-5x1MUnits 5x1MUnits
EUR 1865

Hygromycin B Solution
09287-84 20ML
EUR 107.8

HYGROMYCIN B SOLUTION
30-240-CR 20 mL/pk
EUR 188.4
Description: Media Catalog; Cell Culture Reagents

Hygromycin B Antibody
abx342220-100l 100 µl
EUR 4500

Hygromycin B Antibody
abx342220-50l 50 µl
EUR 900

EZSolution? Hygromycin B
2590-20 each
EUR 326.4

Hygromycin B (HGR), 90%
67317 100 Mg
EUR 10.58
Description: Part B

HYGROMYCIN B 1000 UNIT POWDER
H397 1G
EUR 259.3

Hygromycin B Concentrated
H001-1MU 1 MU
EUR 58.67
Description: C20H37N3O13

Hygromycin antibody
Hygromycin Phosphotrans.mAb
MBS834411-1mg 1mg
EUR 940

Hygromycin antibody
Hygromycin Phosphotrans.mAb
MBS834411-5x1mg 5x1mg
EUR 4085

Hygromycin B EvoPure®
H010-1G 1 g
EUR 141.32
Description: C20H37N3O13

Hygromycin B EvoPure®
H010-500MG 500 mg
EUR 101.88
Description: C20H37N3O13

HYGROMYCIN B (100 mg/mL WATER)
H385 10ML
EUR 121.53

Hygromycin B::BSA Conjugate
40810006-1 5 mg
EUR 198.58

Hygromycin B::BSA Conjugate
40810006-2 20 mg
EUR 354.14

Hygromycin B::KLH Conjugate
40810007-1 5 mg
EUR 215.83

Hygromycin B::KLH Conjugate
40810007-2 20 mg
EUR 354.14

Hygromycin B Gold (solution)
ant-hg-1 1 g (10 x 1 ml)
EUR 144.9

Hygromycin B Gold (solution)
ant-hg-2 2 g (20 x 1 ml)
EUR 248.85

Hygromycin B Gold (solution)
ant-hg-5 5 g (1 x 50 ml)
EUR 460.95

HYGROMYCIN B (50 mg/mL PBS)
H370 20ML
EUR 121.53

50mg/mL Hygromycin B Solution
PB180126-10mL 10 mL
EUR 88
Description: Supplements & Reagents

50mg/mL Hygromycin B Solution
PB180126 10mL
EUR 88
Description: Antibiotics

Hygromycin B 50mg/ml Solution
MBS651334-10x1mL 10x1mL
EUR 395

Hygromycin B 50mg/ml Solution
MBS651334-1mL 1mL
EUR 160

Hygromycin B 50mg/ml Solution
MBS651334-5x1mL 5x1mL
EUR 285

50mg/mL Hygromycin B Solution
MBS2570243-10mL 10mL
EUR 145

50mg/mL Hygromycin B Solution
MBS2570243-5x10mL 5x10mL
EUR 635

Hygromycin B Deuterated (d4 major)
H997604 10mg
EUR 1471

Hygromycin B EvoPure (Low Endotoxin)
H024-1G 1g
EUR 456.05
Description: C20H37N3O13

Hygromycin B EvoPure (Low Endotoxin)
H024-500MG 500 mg
EUR 281.78
Description: C20H37N3O13

Hygromycin B Solution (Low Endotoxin)
H026-20ML 20 mL
EUR 77.55
Description: C20H37N3O13

Hygromycin Antibody
abx023698-1mg 1 mg
EUR 1312.8

Hygromycin Antibody
abx023699-1mg 1 mg
EUR 1312.8

Hygromycin Solution
Hygro-anti 1.0 ml (50 mg/ml)
EUR 70
Description: Hygromycin antibiotic selection solution in PBS

Hygromycin antibody
MBS832031-1mg 1mg
EUR 940

Hygromycin antibody
MBS832031-5x1mg 5x1mg
EUR 4085

Hygromycin antibody
MBS832436-1mg 1mg
EUR 940

Hygromycin antibody
MBS832436-5x1mg 5x1mg
EUR 4085

Hygromycin antibody
MBS833411-1mg 1mg
EUR 940

Hygromycin antibody
MBS833411-5x1mg 5x1mg
EUR 4085

Hygromycin B Solution, 50mg/mL, Sterile
40810004-1 25 mL
EUR 80.99

Hygromycin B Solution, 50mg/mL, Sterile
40810004-2 50 mL
EUR 150.08

Hygromycin B Solution, 100mg/mL, Sterile
40810005-1 10 mL
EUR 94.19

Hygromycin B, Solution (50 mg/ml) - 10 ml
CAHYG-H each
EUR 100.48

Hygromycin B (HGR) for tissue culture, 90%
11056 100 Mg
EUR 18.45
Description: Part B

Hygromycin B Solution (50 mg/ml in PBS Buffer)
H011-20ML 20 mL
EUR 70.47
Description: C20H37N3O13

Hygromycin B Solution (50 mg/ml in PBS Buffer)
H011-5x20ML 5x20mL
EUR 291.99
Description: C20H37N3O13

Escherichia coli Hygromycin-B 4-O-kinase (hph)
1-CSB-YP365448ENL
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in Yeast

Escherichia coli Hygromycin-B 4-O-kinase (hph)
1-CSB-EP365448ENL
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in E.coli

Escherichia coli Hygromycin-B 4-O-kinase (hph)
1-CSB-EP365448ENLa0
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in E.coli

Hygromycin B (HGR Solution), 50mg/ml in Aq. Solution
94663 20 ml
EUR 73.5
Description: Part B

Enhanced GFP Lentivirus (Hygromycin)
78639-H 500 µl x 2
EUR 835
Description: Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased fluorescence and more efficient folding. The Enhanced GFP Lentivirus are replication-incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express eGFP under a CMV promoter. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.

Hygromycin phosphotransferase antibody
10-1418 1 mg
EUR 600
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody
10-1419 1 mg
EUR 600
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody
10-1420 1 mg
EUR 600
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody
10-1421 1 mg
EUR 600
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Cas9 Lentivirus (Hygromycin Selection)
78067 500 µl x 2
EUR 795
Description: Cas9 Lentivirus can be used to generate Cas9 expressing cells in almost any mammalian cell line. Cells stably expressing Cas9 can then be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.

The Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a Cas9 gene driven by an EF1a promoter, along with a Hygromycin selection marker._x000D_

Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer)
H015-10x2ML 10 x 2 mL
EUR 225.86
Description: C20H37N3O13

Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer)
H015-5x2ML 5 x 2 mL
EUR 141.32
Description: C20H37N3O13

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS1233003-002mgEColi 0.02mg(E-Coli)
EUR 375

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS1233003-002mgYeast 0.02mg(Yeast)
EUR 420

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS1233003-01mgEColi 0.1mg(E-Coli)
EUR 635

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS1233003-01mgYeast 0.1mg(Yeast)
EUR 695

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS1233003-05mgYeast 0.5mg(Yeast)
EUR 1505

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS9421057-002mg 0.02mg
EUR 460

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS9421057-01mg 0.1mg
EUR 795

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS9421057-1mg 1mg
EUR 2525

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS9421057-5x1mg 5x1mg
EUR 11205

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS9422697-002mg 0.02mg
EUR 505

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS9422697-01mg 0.1mg
EUR 895

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS9422697-05mg 0.5mg
EUR 1975

Recombinant Escherichia coli Hygromycin-B 4-O-kinase
MBS9422697-5x05mg 5x0.5mg
EUR 8735

Firefly Luciferase Lentivirus Hygromycin
79692-H 500 µl x 2
EUR 875
Description: The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses constitutively express firefly luciferase under a CMV promoter.

MEF Feeder Cells (Hygromycin-resistant)
CBA-313 1 vial
EUR 553.2
Description: MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Hygromycin resistant.

Hygromycin Phosphotransferase (HPT) Antibody
abx018357-1mg 1 mg
EUR 512.5

Hygromycin Phosphotransferase (HPT) Antibody
abx018358-1mg 1 mg
EUR 512.5

Hygromycin Phosphotransferase (HPT) Antibody
abx018359-1mg 1 mg
EUR 512.5

Hygromycin Phosphotransferase (HPT) Antibody
abx018360-2ml 2 ml
EUR 512.5

Recombinant Streptomyces hygroscopicus Hygromycin-B 7'-O-kinase (hyg)
MBS1110975-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1210

Recombinant Streptomyces hygroscopicus Hygromycin-B 7'-O-kinase (hyg)
MBS1110975-002mgEColi 0.02mg(E-Coli)
EUR 860

Recombinant Streptomyces hygroscopicus Hygromycin-B 7'-O-kinase (hyg)
MBS1110975-002mgMammalianCell 0.02mg(Mammalian-Cell)
EUR 1475

Recombinant Streptomyces hygroscopicus Hygromycin-B 7'-O-kinase (hyg)
MBS1110975-01mgBaculovirus 0.1mg(Baculovirus)
EUR 1735

Recombinant Streptomyces hygroscopicus Hygromycin-B 7'-O-kinase (hyg)
MBS1110975-01mgEColi 0.1mg(E-Coli)
EUR 1035

EF1a-(Red-Luciferase) Lentivirus (Hygromycin)
LVP1305 1x10^7 IFU/ml x 200ul
EUR 245
Description: Red-Luciferease expression lentivirus under EF1a promoter, containing Hygromycin selection.

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)
CSB-YP365448ENL 6406 mg Ask for price

Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph)
AP71180 1mg
EUR 2826

Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph)
AP72994 each Ask for price

Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph)
AP77479 each Ask for price

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)
CSB-EP365448ENL 3454 mg Ask for price

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)
CSB-EP365448ENLa0 3455 mg Ask for price

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)
RPC20138-100ug 100ug
EUR 721.6

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)
RPC20138-1mg 1mg
EUR 2884.7

We identify PIK3CA, RHOA, MAPK3, PAFAY1B1, CTNNB1, MYC, PRKCA, ERBB2, and CDC42 as a key gene on the MIR-375 network which is set and estimates the possible function of the MIR-375 in the heart muscle, which consists mainly in regulations on signaling paths that are dependent on on RHO-GTPases. We apply our algorithm to predict Mirna’s function into the python module, which is available at GitHub.

Douglas

Leave A Comment