Identification of a miRNA based model to detect prognostic subgroups in patients with aggressive B-cell lymphoma
To distinguish prognostic subgroups patients with aggressive B cell lymphoma, we analyzed 800 MIRNAS’s expression with Nanostring NCounter Micna Mirna Test on cohort 228 FFPE samples of patients registered in the Ricover-60 trial and megachoep. We identify significant Mirna signatures for the overall survival (OS) (OS) and progressive free survival (PFS) with linear cox regression punished by Laso. The level of high expression of the MIR-130A-3P and MIR-423-5P shows a better prognosis, while the high level of MIR-374B-5P, MIR-590-5P, MIR-186-5P, and MIR-106B-5P-5P increases Patients’ risk level for OS.
Regarding the PFS high expression MIR-365A-5P In addition to two other MIRNAS increases the prognosis and high level of MIR374A-5P, MIR-106B-5P, and MIR-590-5P, connected with an increased risk and poor prognosis. We identified the signature of Mirna to divide patients into two different risk groups. This prognostic model can be used in risk stratification in clinical trials in the future and helps make personalized therapeutic decisions.
Comparative analysis of transcript profile, proteome, and mirna cupffer cells and monocytes
The macrophage population in most mammalian organs consists of different origin cells. The macrophage of the population comes from the erythromiseloid precursor of the wall of the egg yolk bag; Maintenance of the amount of macrophages in the postnatal ontogenesis is practically independent of the hematopoiesis bone marrow. The largest population of the population of embryonic population is found in the central nervous system (Mikroglia) and the liver (Cell Cupffer). Conversely, dermis and mucous membranes become largely colonized by monocytes of bone marrow derivatives which show clear and phenotical plasticity.
In this study, we compare cupffer cells and monocytes using immunofenotype, gene expression profiles, proteome, and microrna collections. The observed difference does not consider the macrophage of the residents’ hearts as pure m2 macrophages or the situation owned by a monocyte has a pure M1 feature. Monocytes show signs of high plasticity and sensitivity to the pattern of molecules related to pathogens (for example, high transcription rates for <i> TLR 2 </ i>, <i> 4 </ i>, <i> 7 </ i>, and <i> 8 </ i>). Conversely, the macrophage of the Heart of the population is clearly involved in the regulations of certain organ functions (nitrogen metabolism, a complementary system protein synthesis).
PMDNE: Prediction of the Mirna-Disease Association based on network embedding and network similarity analysis
Prediction of success from the Mirna-Disease Association is nontrivial for the diagnosis and prognosis of genetic diseases. There are many methods for predicting mirna and disease, but many biological data and complex, and they are often in network form. Ways to accurately use MIRNA features and biological tissue related to diseases to predict unknown associations always become a challenge. Here, we propose PMDNE, a method based on network embedding and network similarity analysis, to predict Mirna’s associations.
At PMDNE, the structure of bipartite graphics network is improved, and the generator runs randomly designed. For embedded vectors, 128 dimensions are used, and predictive accuracy increases significantly. Compared to other network embedding methods, PMDNE is comparable and competitive with the method of art conditions. The method we can solve features extraction features, reduce the dimensions of features, and increase the efficiency of predictions of mirna-disease associations. This method can also be extended to other areas for biomedical tissue predictions.
Effect of lifestyle factors on mirna expression and signal paths: reviews
The term ‘lifestyle’ includes various factors that contribute to maintenance of good health status. Increased evidence shows that lifestyle factors can affect epigenetic mechanisms, such as MIRNAS’s expression. MIRNA dysregulation can modify gene expression and molecular pathways that can cause functional changes. This review summarizes human studies that highlights that diet, physical activity, smoking consumption and alcohol can affect the Mirna engine and some biological functions.
Most Mirnas are involved in molecular lines that affect inflammation, cell cycle regulations and carcinogenesis produce onset or the development of pathological conditions. Investigating this interaction will be very important to understand the etiology of the pathological process, the potential for new care strategies and to prevent disease.
From the Mirna target network to the Mirna function: MIR-375 can regulate apoptosis and dynamics of actin in the heart muscle through the path that depends on RHO-GTPASES
Micrornas (MIRNAS) is a short ribonucleic acid molecule (RNA), without code, which is involved in the main biological process regulations, such as apoptosis or proliferation and cell differentiation, through specific interactions of sequences with the MRNAS target. In this study, we propose workflows to predict the MIRNA function by analyzing their target gene network structure. This workflow is applied to study the functional role of the MIR-375 in the heart of the muscle (myocardium), because Mirna previously proved to be associated with heart disease, and the data on its functions in myocardium was largely unclear.
Hygromycin B |
H007-1G |
TOKU-E |
1 g |
EUR 120 |
Hygromycin B |
H007-500MG |
TOKU-E |
500 mg |
EUR 81.6 |
Hygromycin B |
H007-5x1G |
TOKU-E |
5 x 1 g |
EUR 384 |
Hygromycin B |
40810000-1 |
Bio-WORLD |
50 mg |
EUR 26.54 |
|
Hygromycin B |
40810000-2 |
Bio-WORLD |
100 mg |
EUR 35.69 |
|
Hygromycin B |
40810000-3 |
Bio-WORLD |
250 mg |
EUR 53.99 |
|
Hygromycin B |
40810000-4 |
Bio-WORLD |
1 g |
EUR 67.81 |
|
Hygromycin B |
40810000-5 |
Bio-WORLD |
5 g |
EUR 255.31 |
|
Hygromycin B |
40810000-8 |
Bio-WORLD |
25 g |
EUR 905.02 |
|
Hygromycin B |
40810000-9 |
Bio-WORLD |
10 g |
EUR 493.23 |
|
Hygromycin B, 1g |
R3128-1g |
ACTGene |
each |
EUR 362.4 |
Hygromycin B, 10g |
R3128-10g |
ACTGene |
each |
EUR 2724 |
Hygromycin B, 10g |
R3128 |
ACTGene |
10g |
EUR 1918.7 |
|
HYGROMYCIN B SOLUTION |
30-240-CR |
CORNING |
20 mL/pk |
EUR 188.4 |
Description: Media Catalog; Cell Culture Reagents |
EZSolution? Hygromycin B |
2590-20 |
Biovision |
each |
EUR 326.4 |
Hygromycin B Concentrated |
H001-1MU |
TOKU-E |
1 MU |
EUR 116.4 |
Hygromycin B EvoPure® |
H010-1G |
TOKU-E |
1 g |
EUR 225.6 |
Hygromycin B EvoPure® |
H010-500MG |
TOKU-E |
500 mg |
EUR 172.8 |
Hygromycin B::BSA Conjugate |
40810006-1 |
Bio-WORLD |
5 mg |
EUR 198.58 |
Hygromycin B::BSA Conjugate |
40810006-2 |
Bio-WORLD |
20 mg |
EUR 354.14 |
Hygromycin B::KLH Conjugate |
40810007-1 |
Bio-WORLD |
5 mg |
EUR 215.83 |
Hygromycin B::KLH Conjugate |
40810007-2 |
Bio-WORLD |
20 mg |
EUR 354.14 |
50mg/mL Hygromycin B Solution |
PB180126-10mL |
Elabscience Biotech |
10 mL |
EUR 88 |
Description: Supplements & Reagents |
Hygromycin B EvoPure (Low Endotoxin) |
H024-1G |
TOKU-E |
1g |
EUR 650.4 |
Hygromycin B EvoPure (Low Endotoxin) |
H024-500MG |
TOKU-E |
500 mg |
EUR 415.2 |
Hygromycin B Solution (Low Endotoxin) |
H026-20ML |
TOKU-E |
20 mL |
EUR 142.8 |
Hygromycin Antibody |
abx023698-1mg |
Abbexa |
1 mg |
EUR 1312.8 |
|
Hygromycin Antibody |
abx023699-1mg |
Abbexa |
1 mg |
EUR 1312.8 |
|
Hygromycin B Solution, 50mg/mL, Sterile |
40810004-1 |
Bio-WORLD |
25 mL |
EUR 80.99 |
|
Hygromycin B Solution, 50mg/mL, Sterile |
40810004-2 |
Bio-WORLD |
50 mL |
EUR 150.08 |
|
Hygromycin B Solution, 100mg/mL, Sterile |
40810005-1 |
Bio-WORLD |
10 mL |
EUR 94.19 |
|
Hygromycin B Solution (50 mg/ml in PBS Buffer) |
H011-20ML |
TOKU-E |
20 mL |
EUR 133.2 |
Hygromycin B Solution (50 mg/ml in PBS Buffer) |
H011-5x20ML |
TOKU-E |
5x20mL |
EUR 440.4 |
Escherichia coli Hygromycin-B 4-O-kinase (hph) |
1-CSB-EP365448ENL |
Cusabio |
-
EUR 733.20
-
EUR 370.80
-
EUR 2192.40
-
EUR 1126.80
-
EUR 1461.60
-
EUR 476.40
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in E.coli |
Escherichia coli Hygromycin-B 4-O-kinase (hph) |
1-CSB-EP365448ENLa0 |
Cusabio |
-
EUR 733.20
-
EUR 370.80
-
EUR 2192.40
-
EUR 1126.80
-
EUR 1461.60
-
EUR 476.40
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in E.coli |
Escherichia coli Hygromycin-B 4-O-kinase (hph) |
1-CSB-YP365448ENL |
Cusabio |
-
EUR 814.80
-
EUR 402.00
-
EUR 2606.40
-
EUR 1261.20
-
EUR 1730.40
-
EUR 522.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in Yeast |
Enhanced GFP Lentivirus (Hygromycin) |
78639-H |
BPS Bioscience |
500 µl x 2 |
EUR 835 |
Description: Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased fluorescence and more efficient folding. The Enhanced GFP Lentivirus are replication-incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express eGFP under a CMV promoter. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1. |
Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer) |
H015-10x2ML |
TOKU-E |
10 x 2 mL |
EUR 340.8 |
Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer) |
H015-5x2ML |
TOKU-E |
5 x 2 mL |
EUR 225.6 |
Cas9 Lentivirus (Hygromycin Selection) |
78067 |
BPS Bioscience |
500 µl x 2 |
EUR 795 |
Description: Cas9 Lentivirus can be used to generate Cas9 expressing cells in almost any mammalian cell line. Cells stably expressing Cas9 can then be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
The Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a Cas9 gene driven by an EF1a promoter, along with a Hygromycin selection marker._x000D_ |
Hygromycin phosphotransferase antibody |
10-1418 |
Fitzgerald |
1 mg |
EUR 813.6 |
Description: Mouse monoclonal Hygromycin phosphotransferase antibody |
Hygromycin phosphotransferase antibody |
10-1419 |
Fitzgerald |
1 mg |
EUR 813.6 |
Description: Mouse monoclonal Hygromycin phosphotransferase antibody |
Hygromycin phosphotransferase antibody |
10-1420 |
Fitzgerald |
1 mg |
EUR 813.6 |
Description: Mouse monoclonal Hygromycin phosphotransferase antibody |
Hygromycin phosphotransferase antibody |
10-1421 |
Fitzgerald |
1 mg |
EUR 813.6 |
Description: Mouse monoclonal Hygromycin phosphotransferase antibody |
Firefly Luciferase Lentivirus Hygromycin |
79692-H |
BPS Bioscience |
500 µl x 2 |
EUR 875 |
Description: The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses constitutively express firefly luciferase under a CMV promoter. |
MEF Feeder Cells (Hygromycin-resistant) |
CBA-313 |
Cell Biolabs |
1 vial |
EUR 553.2 |
Description: MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Hygromycin resistant. |
Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph) |
RPC20138-100ug |
Biomatik Corporation |
100ug |
EUR 721.6 |
Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph) |
RPC20138-1mg |
Biomatik Corporation |
1mg |
EUR 2884.7 |
Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph) |
RPC20138-20ug |
Biomatik Corporation |
20ug |
EUR 448.1 |
Expression Negative Control Lentivirus (Hygromycin) |
79902-H |
BPS Bioscience |
500 µl x 2 |
EUR 795 |
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_ |
TCR Activator Lentivirus (EF1a Promoter/Hygromycin) |
79894-EH |
BPS Bioscience |
500 µl x 2 |
EUR 795 |
Description: The TCR Activator Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a gene for a membrane-bound, engineered T cell receptor (TCR) activator driven by a CMV promoter or an EF1a promoter. After transduction, the expression of TCR activator can be monitored in the target cells. |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-1mg |
QP7397-ye-1mg |
EnQuireBio |
1mg |
EUR 3296.4 |
Murashige and Skoog (MS) Medium Plates, w/ Hygromycin |
30630060-1 |
Bio-WORLD |
20 Plate(s) |
EUR 84.23 |
Murashige and Skoog (MS) Medium Plates, w/ Hygromycin |
30630060-2 |
Bio-WORLD |
50 Plate(s) |
EUR 173.72 |
Murashige and Skoog (MS) Medium Plates, w/ Hygromycin |
30630060-3 |
Bio-WORLD |
25 Plate(s) |
EUR 84.23 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-10ug |
QP7397-ye-10ug |
EnQuireBio |
10ug |
EUR 434.4 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-50ug |
QP7397-ye-50ug |
EnQuireBio |
50ug |
EUR 576 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-100ug |
QP7397-ye-100ug |
EnQuireBio |
100ug |
EUR 945.6 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-200ug |
QP7397-ye-200ug |
EnQuireBio |
200ug |
EUR 1512 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-500ug |
QP7397-ye-500ug |
EnQuireBio |
500ug |
EUR 2164.8 |
LEXSY Hygro, Hygromycin B, 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service |
M-AB-104S |
MiTeGen |
1 ml |
EUR 86 |
Description: LEXSY Hygro, Hygromycin B, 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-1mg |
QP7397-ec-1mg |
EnQuireBio |
1mg |
EUR 2763.6 |
LEXSY Hygro, Hygromycin B, 5 x 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service |
M-AB-104L |
MiTeGen |
5 x 1 ml |
EUR 294 |
Description: LEXSY Hygro, Hygromycin B, 5 x 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-100ug |
QP7397-ec-100ug |
EnQuireBio |
100ug |
EUR 848.4 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-10ug |
QP7397-ec-10ug |
EnQuireBio |
10ug |
EUR 391.2 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-200ug |
QP7397-ec-200ug |
EnQuireBio |
200ug |
EUR 1338 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-500ug |
QP7397-ec-500ug |
EnQuireBio |
500ug |
EUR 1816.8 |
Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-50ug |
QP7397-ec-50ug |
EnQuireBio |
50ug |
EUR 522 |
SEAP (EF1a, Hygro) |
LVP1200 |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 418.8 |
Description: Lentivirus express SEAP under EF1a promoter, containing Hygromycin selection. |
SEAP (EF1a, Hygro) in PBS |
LVP1200-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Hygromycin selection. |
Hygrolidin |
H033-1MG |
TOKU-E |
1 mg |
EUR 381.6 |
Hygrolidin |
H033-5MG |
TOKU-E |
5 mg |
EUR 1248 |
SEAP (TetCMV, Hygro) |
LVP1191 |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 418.8 |
Description: Lentivirus express SEAP under optional inducible TetCMV promoter, containing Hygromycin selection. |
SEAP (TetCMV, Hygro) in PBS |
LVP1191-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Hygromycin selection. |
HEK293-TetR (Hygro) |
SC005-Hygro |
GenTarget |
2 x 106 cell/ml x 1ml |
EUR 1452 |
Description: tetracycline repressor in HEK293 stable cell line with Hygromycin fusion marker |
CMV control lentivirus (Hygro) |
CMV-Null-Hygro |
GenTarget |
1 x107 IFU/ml x 200ul |
EUR 418.8 |
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter. |
EF1a control lentivirus (Hygro) |
EF1a-Null-Hygro |
GenTarget |
1 x107 IFU/ml x 200ul |
EUR 418.8 |
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter. |
Recombinant S. hygroscopicus BAR |
EPT071 |
ELK Biotech |
50ug |
EUR 353.4 |
|
Description: Recombinant Streptomyces Hygroscopicus Phosphinothricin N-acetyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed. |
CMV-TetR (Hygro) Lentiviral particles |
LVP017-Hygro |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 484.8 |
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Hygromycin antibiotic marker. |
EF1a-TetR (Hygro) Lentiviral particles |
LVP459-Hygro |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 484.8 |
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhaced EF1a promoter, with Hygromycin antibiotic marker. |
CMV-TetR (Hygro) Lentiviral particles in PBS |
LVP017-Hygro-PBS |
GenTarget |
5x107 IFU/ml x 200ul |
EUR 608.4 |
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Hygromycin antibiotic marker. Particles pellet was re-suspended in PBS solution. |
EF1a-TetR (Hygro) Lentiviral particles in PBS |
LVP459-Hygro-PBS |
GenTarget |
5x107 IFU/ml x 200ul |
EUR 608.4 |
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under Enahnced EF1a promoter, with Hygromycin antibiotic marker. Particles pellet was re-suspended in PBS solution. |
AAV-1 Helper Free Bicistronic Expression System (Hygro) |
VPK-417-SER1 |
Cell Biolabs |
1 kit |
EUR 1486.8 |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
AAV-2 Helper Free Bicistronic Expression System (Hygro) |
VPK-417-SER2 |
Cell Biolabs |
1 kit |
EUR 1486.8 |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
AAV-3 Helper Free Bicistronic Expression System (Hygro) |
VPK-417-SER3 |
Cell Biolabs |
1 kit |
EUR 1486.8 |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
AAV-4 Helper Free Bicistronic Expression System (Hygro) |
VPK-417-SER4 |
Cell Biolabs |
1 kit |
EUR 1486.8 |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
AAV-5 Helper Free Bicistronic Expression System (Hygro) |
VPK-417-SER5 |
Cell Biolabs |
1 kit |
EUR 1486.8 |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
AAV-6 Helper Free Bicistronic Expression System (Hygro) |
VPK-417-SER6 |
Cell Biolabs |
1 kit |
EUR 1486.8 |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). |
AAV-DJ Helper Free Bicistronic Expression System (Hygro) |
VPK-417-DJ |
Cell Biolabs |
1 kit |
EUR 1486.8 |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. |
AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro) |
VPK-417-DJ-8 |
Cell Biolabs |
1 kit |
EUR 1486.8 |
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues. |
ViraSafe Lentiviral Expression System (Hygro), Ecotropic |
VPK-214-ECO |
Cell Biolabs |
1 kit |
EUR 1395.6 |
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures. |
ViraSafe Lentiviral Expression System (Hygro), Pantropic |
VPK-214-PAN |
Cell Biolabs |
1 kit |
EUR 1395.6 |
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin. |
ViraSafe Lentiviral Bicistronic Expression System (Hygro), Ecotropic |
VPK-217-ECO |
Cell Biolabs |
1 kit |
EUR 1395.6 |
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures. |
ViraSafe Lentiviral Bicistronic Expression System (Hygro), Pantropic |
VPK-217-PAN |
Cell Biolabs |
1 kit |
EUR 1395.6 |
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin. |
Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar) |
1-CSB-YP321441SOO |
Cusabio |
-
EUR 814.80
-
EUR 402.00
-
EUR 2606.40
-
EUR 1261.20
-
EUR 1730.40
-
EUR 522.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase(bar) expressed in Yeast |
Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar) |
1-CSB-EP321441SOO |
Cusabio |
-
EUR 733.20
-
EUR 370.80
-
EUR 2192.40
-
EUR 1126.80
-
EUR 1461.60
-
EUR 476.40
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase(bar) expressed in E.coli |
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar |
CM65-10ug |
Novoprotein |
10ug |
EUR 187.2 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar |
CM65-1mg |
Novoprotein |
1mg |
EUR 2739.6 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar |
CM65-500ug |
Novoprotein |
500ug |
EUR 1935.6 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar |
CM65-50ug |
Novoprotein |
50ug |
EUR 442.8 |
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar) |
RPC20112-100ug |
Biomatik Corporation |
100ug |
EUR 721.6 |
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar) |
RPC20112-1mg |
Biomatik Corporation |
1mg |
EUR 2884.7 |
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar) |
RPC20112-20ug |
Biomatik Corporation |
20ug |
EUR 448.1 |
Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/bar Protein, No Tag |
E40PO147 |
EnoGene |
20ug |
EUR 495 |
We identify PIK3CA, RHOA, MAPK3, PAFAY1B1, CTNNB1, MYC, PRKCA, ERBB2, and CDC42 as a key gene on the MIR-375 network which is set and estimates the possible function of the MIR-375 in the heart muscle, which consists mainly in regulations on signaling paths that are dependent on on RHO-GTPases. We apply our algorithm to predict Mirna’s function into the python module, which is available at GitHub.