Identification of a miRNA based model to detect prognostic subgroups in patients with aggressive B-cell lymphoma

To distinguish prognostic subgroups patients with aggressive B cell lymphoma, we analyzed 800 MIRNAS’s expression with Nanostring NCounter Micna Mirna Test on cohort 228 FFPE samples of patients registered in the Ricover-60 trial and megachoep. We identify significant Mirna signatures for the overall survival (OS) (OS) and progressive free survival (PFS) with linear cox regression punished by Laso. The level of high expression of the MIR-130A-3P and MIR-423-5P shows a better prognosis, while the high level of MIR-374B-5P, MIR-590-5P, MIR-186-5P, and MIR-106B-5P-5P increases Patients’ risk level for OS.

Regarding the PFS high expression MIR-365A-5P In addition to two other MIRNAS increases the prognosis and high level of MIR374A-5P, MIR-106B-5P, and MIR-590-5P, connected with an increased risk and poor prognosis. We identified the signature of Mirna to divide patients into two different risk groups. This prognostic model can be used in risk stratification in clinical trials in the future and helps make personalized therapeutic decisions.

Comparative analysis of transcript profile, proteome, and mirna cupffer cells and monocytes

The macrophage population in most mammalian organs consists of different origin cells. The macrophage of the population comes from the erythromiseloid precursor of the wall of the egg yolk bag; Maintenance of the amount of macrophages in the postnatal ontogenesis is practically independent of the hematopoiesis bone marrow. The largest population of the population of embryonic population is found in the central nervous system (Mikroglia) and the liver (Cell Cupffer). Conversely, dermis and mucous membranes become largely colonized by monocytes of bone marrow derivatives which show clear and phenotical plasticity.

In this study, we compare cupffer cells and monocytes using immunofenotype, gene expression profiles, proteome, and microrna collections. The observed difference does not consider the macrophage of the residents’ hearts as pure m2 macrophages or the situation owned by a monocyte has a pure M1 feature. Monocytes show signs of high plasticity and sensitivity to the pattern of molecules related to pathogens (for example, high transcription rates for <i> TLR 2 </ i>, <i> 4 </ i>, <i> 7 </ i>, and <i> 8 </ i>). Conversely, the macrophage of the Heart of the population is clearly involved in the regulations of certain organ functions (nitrogen metabolism, a complementary system protein synthesis).

PMDNE: Prediction of the Mirna-Disease Association based on network embedding and network similarity analysis

Prediction of success from the Mirna-Disease Association is nontrivial for the diagnosis and prognosis of genetic diseases. There are many methods for predicting mirna and disease, but many biological data and complex, and they are often in network form. Ways to accurately use MIRNA features and biological tissue related to diseases to predict unknown associations always become a challenge. Here, we propose PMDNE, a method based on network embedding and network similarity analysis, to predict Mirna’s associations.

At PMDNE, the structure of bipartite graphics network is improved, and the generator runs randomly designed. For embedded vectors, 128 dimensions are used, and predictive accuracy increases significantly. Compared to other network embedding methods, PMDNE is comparable and competitive with the method of art conditions. The method we can solve features extraction features, reduce the dimensions of features, and increase the efficiency of predictions of mirna-disease associations. This method can also be extended to other areas for biomedical tissue predictions.

Effect of lifestyle factors on mirna expression and signal paths: reviews

The term ‘lifestyle’ includes various factors that contribute to maintenance of good health status. Increased evidence shows that lifestyle factors can affect epigenetic mechanisms, such as MIRNAS’s expression. MIRNA dysregulation can modify gene expression and molecular pathways that can cause functional changes. This review summarizes human studies that highlights that diet, physical activity, smoking consumption and alcohol can affect the Mirna engine and some biological functions.

Most Mirnas are involved in molecular lines that affect inflammation, cell cycle regulations and carcinogenesis produce onset or the development of pathological conditions. Investigating this interaction will be very important to understand the etiology of the pathological process, the potential for new care strategies and to prevent disease.

From the Mirna target network to the Mirna function: MIR-375 can regulate apoptosis and dynamics of actin in the heart muscle through the path that depends on RHO-GTPASES

Micrornas (MIRNAS) is a short ribonucleic acid molecule (RNA), without code, which is involved in the main biological process regulations, such as apoptosis or proliferation and cell differentiation, through specific interactions of sequences with the MRNAS target. In this study, we propose workflows to predict the MIRNA function by analyzing their target gene network structure. This workflow is applied to study the functional role of the MIR-375 in the heart of the muscle (myocardium), because Mirna previously proved to be associated with heart disease, and the data on its functions in myocardium was largely unclear.

Hygromycin B

AG190 1 mg
EUR 627.6

Hygromycin B

GA7834-100MG 100 mg
EUR 74.4

Hygromycin B

GA7834-1G 1 g
EUR 151.2

Hygromycin B

GA7834-500MG 500 mg
EUR 103.2

Hygromycin B

GA7834-5G 5 g
EUR 495.6

Hygromycin B

HY-B0490 10mM/1mL
EUR 151.2

Hygromycin B

H007-1G 1 g
EUR 120

Hygromycin B

H007-500MG 500 mg
EUR 81.6

Hygromycin B

H007-5x1G 5 x 1 g
EUR 384

Hygromycin B

GA7834-1 1
EUR 95

Hygromycin B

GA7834-100 100
EUR 31.7

Hygromycin B

GA7834-5 5
EUR 379.6

Hygromycin B

GA7834-500 500
EUR 55.4

Hygromycin B

40810000-1 50 mg
EUR 26.54

Hygromycin B

40810000-2 100 mg
EUR 35.69

Hygromycin B

40810000-3 250 mg
EUR 53.99

Hygromycin B

40810000-4 1 g
EUR 67.81

Hygromycin B

40810000-5 5 g
EUR 255.31

Hygromycin B

40810000-8 25 g
EUR 905.02

Hygromycin B

40810000-9 10 g
EUR 493.23

Hygromycin B

A2813-100MG 100MG
EUR 39.6
Description: Cell Cuture

Hygromycin B, 1g

R3128-1g each
EUR 362.4

Hygromycin B [BSA]

DAG4469 0.5mg
EUR 936

Hygromycin B [KLH]

DAG4485 0.5mg
EUR 1248

Hygromycin B, 10g

R3128-10g each
EUR 2724

Hygromycin B, 10g

R3128 10g
EUR 1918.7

HYGROMYCIN B SOLUTION

30-240-CR 20 mL/pk
EUR 188.4
Description: Media Catalog; Cell Culture Reagents

EZSolution? Hygromycin B

2590-20 each
EUR 326.4

Hygromycin B Concentrated

H001-1MU 1 MU
EUR 116.4

Hygromycin B EvoPure®

H010-1G 1 g
EUR 225.6

Hygromycin B EvoPure®

H010-500MG 500 mg
EUR 172.8

Hygromycin B::BSA Conjugate

40810006-1 5 mg
EUR 198.58

Hygromycin B::BSA Conjugate

40810006-2 20 mg
EUR 354.14

Hygromycin B::KLH Conjugate

40810007-1 5 mg
EUR 215.83

Hygromycin B::KLH Conjugate

40810007-2 20 mg
EUR 354.14

50mg/mL Hygromycin B Solution

PB180126-10mL 10 mL
EUR 88
Description: Supplements & Reagents

Hygromycin B EvoPure (Low Endotoxin)

H024-1G 1g
EUR 650.4

Hygromycin B EvoPure (Low Endotoxin)

H024-500MG 500 mg
EUR 415.2

Hygromycin B Solution (Low Endotoxin)

H026-20ML 20 mL
EUR 142.8

Hygromycin Antibody

abx023698-1mg 1 mg
EUR 1312.8

Hygromycin Antibody

abx023699-1mg 1 mg
EUR 1312.8

Hygromycin B Solution, 50mg/mL, Sterile

40810004-1 25 mL
EUR 80.99

Hygromycin B Solution, 50mg/mL, Sterile

40810004-2 50 mL
EUR 150.08

Hygromycin B Solution, 100mg/mL, Sterile

40810005-1 10 mL
EUR 94.19

Hygromycin B Solution (50 mg/ml in PBS Buffer)

H011-20ML 20 mL
EUR 133.2

Hygromycin B Solution (50 mg/ml in PBS Buffer)

H011-5x20ML 5x20mL
EUR 440.4

Escherichia coli Hygromycin-B 4-O-kinase (hph)

1-CSB-EP365448ENL
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in E.coli

Escherichia coli Hygromycin-B 4-O-kinase (hph)

1-CSB-EP365448ENLa0
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in E.coli

Escherichia coli Hygromycin-B 4-O-kinase (hph)

1-CSB-YP365448ENL
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in Yeast

Enhanced GFP Lentivirus (Hygromycin)

78639-H 500 µl x 2
EUR 835
Description: Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased fluorescence and more efficient folding. The Enhanced GFP Lentivirus are replication-incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express eGFP under a CMV promoter. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.

Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer)

H015-10x2ML 10 x 2 mL
EUR 340.8

Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer)

H015-5x2ML 5 x 2 mL
EUR 225.6

Cas9 Lentivirus (Hygromycin Selection)

78067 500 µl x 2
EUR 795
Description: Cas9 Lentivirus can be used to generate Cas9 expressing cells in almost any mammalian cell line. Cells stably expressing Cas9 can then be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.

The Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a Cas9 gene driven by an EF1a promoter, along with a Hygromycin selection marker._x000D_

Hygromycin phosphotransferase antibody

10-1418 1 mg
EUR 813.6
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody

10-1419 1 mg
EUR 813.6
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody

10-1420 1 mg
EUR 813.6
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody

10-1421 1 mg
EUR 813.6
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Firefly Luciferase Lentivirus Hygromycin

79692-H 500 µl x 2
EUR 875
Description: The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses constitutively express firefly luciferase under a CMV promoter.

MEF Feeder Cells (Hygromycin-resistant)

CBA-313 1 vial
EUR 553.2
Description: MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Hygromycin resistant.

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)

RPC20138-100ug 100ug
EUR 721.6

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)

RPC20138-1mg 1mg
EUR 2884.7

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)

RPC20138-20ug 20ug
EUR 448.1

Expression Negative Control Lentivirus (Hygromycin)

79902-H 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

TCR Activator Lentivirus (EF1a Promoter/Hygromycin)

79894-EH 500 µl x 2
EUR 795
Description: The TCR Activator Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a gene for a membrane-bound, engineered T cell receptor (TCR) activator driven by a CMV promoter or an EF1a promoter. After transduction, the expression of TCR activator can be monitored in the target cells.

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-1mg

QP7397-ye-1mg 1mg
EUR 3296.4

Murashige and Skoog (MS) Medium Plates, w/ Hygromycin

30630060-1 20 Plate(s)
EUR 84.23

Murashige and Skoog (MS) Medium Plates, w/ Hygromycin

30630060-2 50 Plate(s)
EUR 173.72

Murashige and Skoog (MS) Medium Plates, w/ Hygromycin

30630060-3 25 Plate(s)
EUR 84.23

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-10ug

QP7397-ye-10ug 10ug
EUR 434.4

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-50ug

QP7397-ye-50ug 50ug
EUR 576

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-100ug

QP7397-ye-100ug 100ug
EUR 945.6

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-200ug

QP7397-ye-200ug 200ug
EUR 1512

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-500ug

QP7397-ye-500ug 500ug
EUR 2164.8

LEXSY Hygro, Hygromycin B, 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service

M-AB-104S 1 ml
EUR 86
Description: LEXSY Hygro, Hygromycin B, 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-1mg

QP7397-ec-1mg 1mg
EUR 2763.6

LEXSY Hygro, Hygromycin B, 5 x 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service

M-AB-104L 5 x 1 ml
EUR 294
Description: LEXSY Hygro, Hygromycin B, 5 x 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-100ug

QP7397-ec-100ug 100ug
EUR 848.4

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-10ug

QP7397-ec-10ug 10ug
EUR 391.2

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-200ug

QP7397-ec-200ug 200ug
EUR 1338

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-500ug

QP7397-ec-500ug 500ug
EUR 1816.8

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-50ug

QP7397-ec-50ug 50ug
EUR 522

ELISA PLATE READER : MICRO READ 1000

1000 1 unit
EUR 2628

ELISA PLATE SHAKER : MICRO SHAKE 1200

1200 1 unit
EUR 916.8

ELISA PLATE WASHER : MICRO WASH 1100

1100 1 unit
EUR 2403.6

Testo 608-1 Hygrometer

HYG4000 EACH
EUR 112.28

Testo 608-2 Hygrometer

HYG4002 EACH
EUR 160.59

Hanna Hygrometer Case

HYG2083 EACH
EUR 81.6

Hanna Thermo Hygrometer HI 9564

HYG2020 EACH
EUR 266.4

Thermometer/hygrometer

DD98463 EACH
EUR 26.4

Digital Thermometer Hygrometer

THE1162 EACH
EUR 28.33

Digital Thermometer Hygrometer

THE1168 EACH
EUR 52.22

pBC- Hygro

PVT5305 2 ug
EUR 390

pLKO.1- Hygro

PVT11107 2 ug
EUR 361.2

SEAP (EF1a, Hygro)

LVP1200 1x107 IFU/ml x 200ul
EUR 418.8
Description: Lentivirus express SEAP under EF1a promoter, containing Hygromycin selection.

pCDNA3.1- Hygro

PVT10675 2 ug
EUR 361.2

pWZL hygro 12S E1A

PVT14501 2 ug
EUR 843.6

Hygrolidin

ADC-P-099 unit Ask for price

SEAP (EF1a, Hygro) in PBS

LVP1200-PBS 1x107 IFU/ml x 200ul
EUR 852
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Hygromycin selection.

Hygrolidin

H033-1MG 1 mg
EUR 381.6

Hygrolidin

H033-5MG 5 mg
EUR 1248

pCDH- CMV- MCS- EF1- Hygro

PVT11076 2 ug
EUR 444

pGL4.15[luc2P Hygro]

PVT17664 2 ug
EUR 360

SEAP (TetCMV, Hygro)

LVP1191 1x107 IFU/ml x 200ul
EUR 418.8
Description: Lentivirus express SEAP under optional inducible TetCMV promoter, containing Hygromycin selection.

pGL4.26[luc2- minP- Hygro]

PVT10798 2 ug
EUR 361.2

pGL4.29[luc2P- CRE- Hygro]

PVT10799 2 ug
EUR 361.2

pGL4.43[Luc29 XPE Hygro]

PVT14287 2 ug
EUR 843.6

pGL4.37[luc2P ARE Hygro]

PVT17453 2 ug
EUR 360

pGL4.42[luc2P HRE Hygro]

PVT12361 2 ug
EUR 843.6

pGL4.47[luc2P SIE Hygro]

PVT12362 2 ug
EUR 843.6

pGL4.48[luc2P SBE Hygro]

PVT12363 2 ug
EUR 843.6

SEAP (TetCMV, Hygro) in PBS

LVP1191-PBS 1x107 IFU/ml x 200ul
EUR 852
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Hygromycin selection.

pGL4.30[luc2P- NFAT- RE- Hygro]

PVT10800 2 ug
EUR 361.2

lenti MS2- P65- HSF1_Hygro

PVT10995 2 ug
EUR 361.2

pGL4.27[luc2P minP Hygro]

PVT17412 2 ug
EUR 409.2

pGL4.39[luc2P?ATF6?RE?Hygro]

PVT12360 2 ug
EUR 843.6

pGL4.52[luc2P STAT5 RE Hygro]

PVT12365 2 ug
EUR 843.6

HEK293-TetR (Hygro)

SC005-Hygro 2 x 106 cell/ml x 1ml
EUR 1452
Description: tetracycline repressor in HEK293 stable cell line with Hygromycin fusion marker

pGL4.32[luc2P/NF-kB-RE/Hygro]

PVT13580 2 ug
EUR 843.6

pGL4.49[luc2P TCF-LEF RE Hygro]

PVT12364 2 ug
EUR 843.6

pGL4.31[luc2P/GAL4UAS/Hygro]

PVT17555 2 ug
EUR 360

pBARGPE1- Hygro Plasmid

PVT5306 2 ug
EUR 390

pGL4.29[luc2P/ CRE/ Hygro] Plasmid

PVT1312 2 ug
EUR 319.2

pGL4.35[luc2P- 9XGAL4UAS- Hygro]

PVT10801 2 ug
EUR 361.2

pSilencer3.1- H1- Stuffer- Hygro

PVT10869 2 ug
EUR 404.4

pBARGPE1- Hygro- EGFP Plasmid

PVT5307 2 ug
EUR 390

CMV control lentivirus (Hygro)

CMV-Null-Hygro 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.

pCAMBIA1303- TrpC- Hygro- gpdA- GFP

PVT11341 2 ug
EUR 444

EF1a control lentivirus (Hygro)

EF1a-Null-Hygro 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.

pcDNA3.1-Hygro-Flag-DRD5 Plasmid

PVTB00688-2a 2 ug
EUR 427.2

Recombinant S. hygroscopicus BAR

EPT071 50ug
EUR 353.4
Description: Recombinant Streptomyces Hygroscopicus Phosphinothricin N-acetyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed.

CMV-TetR (Hygro) Lentiviral particles

LVP017-Hygro 1x107 IFU/ml x 200ul
EUR 484.8
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Hygromycin antibiotic marker.

EF1a-TetR (Hygro) Lentiviral particles

LVP459-Hygro 1x107 IFU/ml x 200ul
EUR 484.8
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhaced EF1a promoter, with Hygromycin antibiotic marker.

CMV-TetR (Hygro) Lentiviral particles  in PBS

LVP017-Hygro-PBS 5x107 IFU/ml x 200ul
EUR 608.4
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Hygromycin antibiotic marker. Particles pellet was re-suspended in PBS solution.

EF1a-TetR (Hygro) Lentiviral particles  in PBS

LVP459-Hygro-PBS 5x107 IFU/ml x 200ul
EUR 608.4
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under Enahnced EF1a promoter, with Hygromycin antibiotic marker. Particles pellet was re-suspended in PBS solution.

AAV-1 Helper Free Bicistronic Expression System (Hygro)

VPK-417-SER1 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-2 Helper Free Bicistronic Expression System (Hygro)

VPK-417-SER2 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-3 Helper Free Bicistronic Expression System (Hygro)

VPK-417-SER3 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-4 Helper Free Bicistronic Expression System (Hygro)

VPK-417-SER4 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-5 Helper Free Bicistronic Expression System (Hygro)

VPK-417-SER5 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-6 Helper Free Bicistronic Expression System (Hygro)

VPK-417-SER6 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-DJ Helper Free Bicistronic Expression System (Hygro)

VPK-417-DJ 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro)

VPK-417-DJ-8 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

ViraSafe Lentiviral Expression System (Hygro), Ecotropic

VPK-214-ECO 1 kit
EUR 1395.6
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Expression System (Hygro), Pantropic

VPK-214-PAN 1 kit
EUR 1395.6
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

ViraSafe Lentiviral Bicistronic Expression System (Hygro), Ecotropic

VPK-217-ECO 1 kit
EUR 1395.6
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Bicistronic Expression System (Hygro), Pantropic

VPK-217-PAN 1 kit
EUR 1395.6
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)

1-CSB-YP321441SOO
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase(bar) expressed in Yeast

Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)

1-CSB-EP321441SOO
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase(bar) expressed in E.coli

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar

CM65-10ug 10ug
EUR 187.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar

CM65-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar

CM65-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar

CM65-50ug 50ug
EUR 442.8
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)

RPC20112-100ug 100ug
EUR 721.6

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)

RPC20112-1mg 1mg
EUR 2884.7

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)

RPC20112-20ug 20ug
EUR 448.1

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/bar Protein, No Tag

E40PO147 20ug
EUR 495

We identify PIK3CA, RHOA, MAPK3, PAFAY1B1, CTNNB1, MYC, PRKCA, ERBB2, and CDC42 as a key gene on the MIR-375 network which is set and estimates the possible function of the MIR-375 in the heart muscle, which consists mainly in regulations on signaling paths that are dependent on on RHO-GTPases. We apply our algorithm to predict Mirna’s function into the python module, which is available at GitHub.

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