To distinguish prognostic subgroups patients with aggressive B cell lymphoma, we analyzed 800 MIRNAS’s expression with Nanostring NCounter Micna Mirna Test on cohort 228 FFPE samples of patients registered in the Ricover-60 trial and megachoep. We identify significant Mirna signatures for the overall survival (OS) (OS) and progressive free survival (PFS) with linear cox regression punished by Laso. The level of high expression of the MIR-130A-3P and MIR-423-5P shows a better prognosis, while the high level of MIR-374B-5P, MIR-590-5P, MIR-186-5P, and MIR-106B-5P-5P increases Patients’ risk level for OS.

Regarding the PFS high expression MIR-365A-5P In addition to two other MIRNAS increases the prognosis and high level of MIR374A-5P, MIR-106B-5P, and MIR-590-5P, connected with an increased risk and poor prognosis. We identified the signature of Mirna to divide patients into two different risk groups. This prognostic model can be used in risk stratification in clinical trials in the future and helps make personalized therapeutic decisions.

Comparative analysis of transcript profile, proteome, and mirna cupffer cells and monocytes

The macrophage population in most mammalian organs consists of different origin cells. The macrophage of the population comes from the erythromiseloid precursor of the wall of the egg yolk bag; Maintenance of the amount of macrophages in the postnatal ontogenesis is practically independent of the hematopoiesis bone marrow. The largest population of the population of embryonic population is found in the central nervous system (Mikroglia) and the liver (Cell Cupffer). Conversely, dermis and mucous membranes become largely colonized by monocytes of bone marrow derivatives which show clear and phenotical plasticity.

In this study, we compare cupffer cells and monocytes using immunofenotype, gene expression profiles, proteome, and microrna collections. The observed difference does not consider the macrophage of the residents’ hearts as pure m2 macrophages or the situation owned by a monocyte has a pure M1 feature. Monocytes show signs of high plasticity and sensitivity to the pattern of molecules related to pathogens (for example, high transcription rates for <i> TLR 2 </ i>, <i> 4 </ i>, <i> 7 </ i>, and <i> 8 </ i>). Conversely, the macrophage of the Heart of the population is clearly involved in the regulations of certain organ functions (nitrogen metabolism, a complementary system protein synthesis).

PMDNE: Prediction of the Mirna-Disease Association based on network embedding and network similarity analysis

Prediction of success from the Mirna-Disease Association is nontrivial for the diagnosis and prognosis of genetic diseases. There are many methods for predicting mirna and disease, but many biological data and complex, and they are often in network form. Ways to accurately use MIRNA features and biological tissue related to diseases to predict unknown associations always become a challenge. Here, we propose PMDNE, a method based on network embedding and network similarity analysis, to predict Mirna’s associations.

At PMDNE, the structure of bipartite graphics network is improved, and the generator runs randomly designed. For embedded vectors, 128 dimensions are used, and predictive accuracy increases significantly. Compared to other network embedding methods, PMDNE is comparable and competitive with the method of art conditions. The method we can solve features extraction features, reduce the dimensions of features, and increase the efficiency of predictions of mirna-disease associations. This method can also be extended to other areas for biomedical tissue predictions.

Effect of lifestyle factors on mirna expression and signal paths: reviews

The term ‘lifestyle’ includes various factors that contribute to maintenance of good health status. Increased evidence shows that lifestyle factors can affect epigenetic mechanisms, such as MIRNAS’s expression. MIRNA dysregulation can modify gene expression and molecular pathways that can cause functional changes. This review summarizes human studies that highlights that diet, physical activity, smoking consumption and alcohol can affect the Mirna engine and some biological functions.

Most Mirnas are involved in molecular lines that affect inflammation, cell cycle regulations and carcinogenesis produce onset or the development of pathological conditions. Investigating this interaction will be very important to understand the etiology of the pathological process, the potential for new care strategies and to prevent disease.

From the Mirna target network to the Mirna function: MIR-375 can regulate apoptosis and dynamics of actin in the heart muscle through the path that depends on RHO-GTPASES

Micrornas (MIRNAS) is a short ribonucleic acid molecule (RNA), without code, which is involved in the main biological process regulations, such as apoptosis or proliferation and cell differentiation, through specific interactions of sequences with the MRNAS target. In this study, we propose workflows to predict the MIRNA function by analyzing their target gene network structure. This workflow is applied to study the functional role of the MIR-375 in the heart of the muscle (myocardium), because Mirna previously proved to be associated with heart disease, and the data on its functions in myocardium was largely unclear.

Hygromycin B
AG190	Unibiotest	1 mg	EUR 627.6

Hygromycin B
GA7834-100MG	Glentham Life Sciences	100 mg	EUR 74.4

Hygromycin B
GA7834-1G	Glentham Life Sciences	1 g	EUR 151.2

Hygromycin B
GA7834-500MG	Glentham Life Sciences	500 mg	EUR 103.2

Hygromycin B
GA7834-5G	Glentham Life Sciences	5 g	EUR 495.6

Hygromycin B
HY-B0490	MedChemExpress	10mM/1mL	EUR 151.2

Hygromycin B
H007-1G	TOKU-E	1 g	EUR 120

Hygromycin B
H007-500MG	TOKU-E	500 mg	EUR 81.6

Hygromycin B
H007-5x1G	TOKU-E	5 x 1 g	EUR 384

Hygromycin B
GA7834-1	Glentham Life Sciences	1	EUR 95

Hygromycin B
GA7834-100	Glentham Life Sciences	100	EUR 31.7

Hygromycin B
GA7834-5	Glentham Life Sciences	5	EUR 379.6

Hygromycin B
GA7834-500	Glentham Life Sciences	500	EUR 55.4

Hygromycin B
40810000-1	Bio-WORLD	50 mg	EUR 26.54

Hygromycin B
40810000-2	Bio-WORLD	100 mg	EUR 35.69

Hygromycin B
40810000-3	Bio-WORLD	250 mg	EUR 53.99

Hygromycin B
40810000-4	Bio-WORLD	1 g	EUR 67.81

Hygromycin B
40810000-5	Bio-WORLD	5 g	EUR 255.31

Hygromycin B
40810000-8	Bio-WORLD	25 g	EUR 905.02

Hygromycin B
40810000-9	Bio-WORLD	10 g	EUR 493.23

Hygromycin B
A2813-100MG	Biomatik Corporation	100MG	EUR 39.6
Description: Cell Cuture

Hygromycin B, 1g
R3128-1g	ACTGene	each	EUR 362.4

Hygromycin B [BSA]
DAG4469	Creative Diagnostics	0.5mg	EUR 936

Hygromycin B [KLH]
DAG4485	Creative Diagnostics	0.5mg	EUR 1248

Hygromycin B, 10g
R3128-10g	ACTGene	each	EUR 2724

Hygromycin B, 10g
R3128	ACTGene	10g	EUR 1918.7

HYGROMYCIN B SOLUTION
30-240-CR	CORNING	20 mL/pk	EUR 188.4
Description: Media Catalog; Cell Culture Reagents

EZSolution? Hygromycin B
2590-20	Biovision	each	EUR 326.4

Hygromycin B Concentrated
H001-1MU	TOKU-E	1 MU	EUR 116.4

Hygromycin B EvoPure®
H010-1G	TOKU-E	1 g	EUR 225.6

Hygromycin B EvoPure®
H010-500MG	TOKU-E	500 mg	EUR 172.8

Hygromycin B::BSA Conjugate
40810006-1	Bio-WORLD	5 mg	EUR 198.58

Hygromycin B::BSA Conjugate
40810006-2	Bio-WORLD	20 mg	EUR 354.14

Hygromycin B::KLH Conjugate
40810007-1	Bio-WORLD	5 mg	EUR 215.83

Hygromycin B::KLH Conjugate
40810007-2	Bio-WORLD	20 mg	EUR 354.14

50mg/mL Hygromycin B Solution
PB180126-10mL	Elabscience Biotech	10 mL	EUR 88
Description: Supplements & Reagents

Hygromycin B EvoPure (Low Endotoxin)
H024-1G	TOKU-E	1g	EUR 650.4

Hygromycin B EvoPure (Low Endotoxin)
H024-500MG	TOKU-E	500 mg	EUR 415.2

Hygromycin B Solution (Low Endotoxin)
H026-20ML	TOKU-E	20 mL	EUR 142.8

Hygromycin Antibody
abx023698-1mg	Abbexa	1 mg	EUR 1312.8

Hygromycin Antibody
abx023699-1mg	Abbexa	1 mg	EUR 1312.8

Hygromycin B Solution, 50mg/mL, Sterile
40810004-1	Bio-WORLD	25 mL	EUR 80.99

Hygromycin B Solution, 50mg/mL, Sterile
40810004-2	Bio-WORLD	50 mL	EUR 150.08

Hygromycin B Solution, 100mg/mL, Sterile
40810005-1	Bio-WORLD	10 mL	EUR 94.19

Hygromycin B Solution (50 mg/ml in PBS Buffer)
H011-20ML	TOKU-E	20 mL	EUR 133.2

Hygromycin B Solution (50 mg/ml in PBS Buffer)
H011-5x20ML	TOKU-E	5x20mL	EUR 440.4

Escherichia coli Hygromycin-B 4-O-kinase (hph)
1-CSB-EP365448ENL	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in E.coli

Escherichia coli Hygromycin-B 4-O-kinase (hph)
1-CSB-EP365448ENLa0	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in E.coli

Escherichia coli Hygromycin-B 4-O-kinase (hph)
1-CSB-YP365448ENL	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph) expressed in Yeast

Enhanced GFP Lentivirus (Hygromycin)
78639-H	BPS Bioscience	500 µl x 2	EUR 835
Description: Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased fluorescence and more efficient folding. The Enhanced GFP Lentivirus are replication-incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. These viruses constitutively express eGFP under a CMV promoter. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.

Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer)
H015-10x2ML	TOKU-E	10 x 2 mL	EUR 340.8

Hygromycin B EvoPure Solution (100 mg/ml in PBS Buffer)
H015-5x2ML	TOKU-E	5 x 2 mL	EUR 225.6

Cas9 Lentivirus (Hygromycin Selection)
78067	BPS Bioscience	500 µl x 2	EUR 795
Description: Cas9 Lentivirus can be used to generate Cas9 expressing cells in almost any mammalian cell line. Cells stably expressing Cas9 can then be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. The Cas9 Lentiviruses are replication incompetent, HIV-based VSV-G pseudo-typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a Cas9 gene driven by an EF1a promoter, along with a Hygromycin selection marker._x000D_

Hygromycin phosphotransferase antibody
10-1418	Fitzgerald	1 mg	EUR 813.6
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody
10-1419	Fitzgerald	1 mg	EUR 813.6
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody
10-1420	Fitzgerald	1 mg	EUR 813.6
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Hygromycin phosphotransferase antibody
10-1421	Fitzgerald	1 mg	EUR 813.6
Description: Mouse monoclonal Hygromycin phosphotransferase antibody

Firefly Luciferase Lentivirus Hygromycin
79692-H	BPS Bioscience	500 µl x 2	EUR 875
Description: The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses constitutively express firefly luciferase under a CMV promoter.

MEF Feeder Cells (Hygromycin-resistant)
CBA-313	Cell Biolabs	1 vial	EUR 553.2
Description: MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Hygromycin resistant.

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)
RPC20138-100ug	Biomatik Corporation	100ug	EUR 721.6

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)
RPC20138-1mg	Biomatik Corporation	1mg	EUR 2884.7

Recombinant Escherichia coli Hygromycin-B 4-O-kinase (hph)
RPC20138-20ug	Biomatik Corporation	20ug	EUR 448.1

Expression Negative Control Lentivirus (Hygromycin)
79902-H	BPS Bioscience	500 µl x 2	EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

TCR Activator Lentivirus (EF1a Promoter/Hygromycin)
79894-EH	BPS Bioscience	500 µl x 2	EUR 795
Description: The TCR Activator Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a gene for a membrane-bound, engineered T cell receptor (TCR) activator driven by a CMV promoter or an EF1a promoter. After transduction, the expression of TCR activator can be monitored in the target cells.

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-1mg
QP7397-ye-1mg	EnQuireBio	1mg	EUR 3296.4

Murashige and Skoog (MS) Medium Plates, w/ Hygromycin
30630060-1	Bio-WORLD	20 Plate(s)	EUR 84.23

Murashige and Skoog (MS) Medium Plates, w/ Hygromycin
30630060-2	Bio-WORLD	50 Plate(s)	EUR 173.72

Murashige and Skoog (MS) Medium Plates, w/ Hygromycin
30630060-3	Bio-WORLD	25 Plate(s)	EUR 84.23

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-10ug
QP7397-ye-10ug	EnQuireBio	10ug	EUR 434.4

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-50ug
QP7397-ye-50ug	EnQuireBio	50ug	EUR 576

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-100ug
QP7397-ye-100ug	EnQuireBio	100ug	EUR 945.6

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-200ug
QP7397-ye-200ug	EnQuireBio	200ug	EUR 1512

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His, Yeast-500ug
QP7397-ye-500ug	EnQuireBio	500ug	EUR 2164.8

LEXSY Hygro, Hygromycin B, 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service
M-AB-104S	MiTeGen	1 ml	EUR 86
Description: LEXSY Hygro, Hygromycin B, 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-1mg
QP7397-ec-1mg	EnQuireBio	1mg	EUR 2763.6

LEXSY Hygro, Hygromycin B, 5 x 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service
M-AB-104L	MiTeGen	5 x 1 ml	EUR 294
Description: LEXSY Hygro, Hygromycin B, 5 x 1 ml (100 mg/ml) ** shipped on blue ice - must be shipped via overnight service

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-100ug
QP7397-ec-100ug	EnQuireBio	100ug	EUR 848.4

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-10ug
QP7397-ec-10ug	EnQuireBio	10ug	EUR 391.2

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-200ug
QP7397-ec-200ug	EnQuireBio	200ug	EUR 1338

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-500ug
QP7397-ec-500ug	EnQuireBio	500ug	EUR 1816.8

Recombinant E.coli Hygromycin-B 4-O-kinase Protein, His-SUMO, E.coli-50ug
QP7397-ec-50ug	EnQuireBio	50ug	EUR 522

ELISA PLATE READER : MICRO READ 1000
1000	Global Diagnostics B	1 unit	EUR 2628

ELISA PLATE SHAKER : MICRO SHAKE 1200
1200	Global Diagnostics B	1 unit	EUR 916.8

ELISA PLATE WASHER : MICRO WASH 1100
1100	Global Diagnostics B	1 unit	EUR 2403.6

Testo 608-1 Hygrometer
HYG4000	Scientific Laboratory Supplies	EACH	EUR 112.28

Testo 608-2 Hygrometer
HYG4002	Scientific Laboratory Supplies	EACH	EUR 160.59

Hanna Hygrometer Case
HYG2083	Scientific Laboratory Supplies	EACH	EUR 81.6

Hanna Thermo Hygrometer HI 9564
HYG2020	Scientific Laboratory Supplies	EACH	EUR 266.4

Thermometer/hygrometer
DD98463	Scientific Laboratory Supplies	EACH	EUR 26.4

Digital Thermometer Hygrometer
THE1162	Scientific Laboratory Supplies	EACH	EUR 28.33

Digital Thermometer Hygrometer
THE1168	Scientific Laboratory Supplies	EACH	EUR 52.22

pBC- Hygro
PVT5305	Lifescience Market	2 ug	EUR 390

pLKO.1- Hygro
PVT11107	Lifescience Market	2 ug	EUR 361.2

SEAP (EF1a, Hygro)
LVP1200	GenTarget	1x107 IFU/ml x 200ul	EUR 418.8
Description: Lentivirus express SEAP under EF1a promoter, containing Hygromycin selection.

pCDNA3.1- Hygro
PVT10675	Lifescience Market	2 ug	EUR 361.2

pWZL hygro 12S E1A
PVT14501	Lifescience Market	2 ug	EUR 843.6

Hygrolidin
ADC-P-099	Creative Biolabs	unit	Ask for price

SEAP (EF1a, Hygro) in PBS
LVP1200-PBS	GenTarget	1x107 IFU/ml x 200ul	EUR 852
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Hygromycin selection.

Hygrolidin
H033-1MG	TOKU-E	1 mg	EUR 381.6

Hygrolidin
H033-5MG	TOKU-E	5 mg	EUR 1248

pCDH- CMV- MCS- EF1- Hygro
PVT11076	Lifescience Market	2 ug	EUR 444

pGL4.15[luc2P Hygro]
PVT17664	Lifescience Market	2 ug	EUR 360

SEAP (TetCMV, Hygro)
LVP1191	GenTarget	1x107 IFU/ml x 200ul	EUR 418.8
Description: Lentivirus express SEAP under optional inducible TetCMV promoter, containing Hygromycin selection.

pGL4.26[luc2- minP- Hygro]
PVT10798	Lifescience Market	2 ug	EUR 361.2

pGL4.29[luc2P- CRE- Hygro]
PVT10799	Lifescience Market	2 ug	EUR 361.2

pGL4.43[Luc29 XPE Hygro]
PVT14287	Lifescience Market	2 ug	EUR 843.6

pGL4.37[luc2P ARE Hygro]
PVT17453	Lifescience Market	2 ug	EUR 360

pGL4.42[luc2P HRE Hygro]
PVT12361	Lifescience Market	2 ug	EUR 843.6

pGL4.47[luc2P SIE Hygro]
PVT12362	Lifescience Market	2 ug	EUR 843.6

pGL4.48[luc2P SBE Hygro]
PVT12363	Lifescience Market	2 ug	EUR 843.6

SEAP (TetCMV, Hygro) in PBS
LVP1191-PBS	GenTarget	1x107 IFU/ml x 200ul	EUR 852
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Hygromycin selection.

pGL4.30[luc2P- NFAT- RE- Hygro]
PVT10800	Lifescience Market	2 ug	EUR 361.2

lenti MS2- P65- HSF1_Hygro
PVT10995	Lifescience Market	2 ug	EUR 361.2

pGL4.27[luc2P minP Hygro]
PVT17412	Lifescience Market	2 ug	EUR 409.2

pGL4.39[luc2P?ATF6?RE?Hygro]
PVT12360	Lifescience Market	2 ug	EUR 843.6

pGL4.52[luc2P STAT5 RE Hygro]
PVT12365	Lifescience Market	2 ug	EUR 843.6

HEK293-TetR (Hygro)
SC005-Hygro	GenTarget	2 x 106 cell/ml x 1ml	EUR 1452
Description: tetracycline repressor in HEK293 stable cell line with Hygromycin fusion marker

pGL4.32[luc2P/NF-kB-RE/Hygro]
PVT13580	Lifescience Market	2 ug	EUR 843.6

pGL4.49[luc2P TCF-LEF RE Hygro]
PVT12364	Lifescience Market	2 ug	EUR 843.6

pGL4.31[luc2P/GAL4UAS/Hygro]
PVT17555	Lifescience Market	2 ug	EUR 360

pBARGPE1- Hygro Plasmid
PVT5306	Lifescience Market	2 ug	EUR 390

pGL4.29[luc2P/ CRE/ Hygro] Plasmid
PVT1312	Lifescience Market	2 ug	EUR 319.2

pGL4.35[luc2P- 9XGAL4UAS- Hygro]
PVT10801	Lifescience Market	2 ug	EUR 361.2

pSilencer3.1- H1- Stuffer- Hygro
PVT10869	Lifescience Market	2 ug	EUR 404.4

pBARGPE1- Hygro- EGFP Plasmid
PVT5307	Lifescience Market	2 ug	EUR 390

CMV control lentivirus (Hygro)
CMV-Null-Hygro	GenTarget	1 x107 IFU/ml x 200ul	EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.

pCAMBIA1303- TrpC- Hygro- gpdA- GFP
PVT11341	Lifescience Market	2 ug	EUR 444

EF1a control lentivirus (Hygro)
EF1a-Null-Hygro	GenTarget	1 x107 IFU/ml x 200ul	EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.

pcDNA3.1-Hygro-Flag-DRD5 Plasmid
PVTB00688-2a	Lifescience Market	2 ug	EUR 427.2

Recombinant S. hygroscopicus BAR
EPT071	ELK Biotech	50ug	EUR 353.4
Description: Recombinant Streptomyces Hygroscopicus Phosphinothricin N-acetyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed.

CMV-TetR (Hygro) Lentiviral particles
LVP017-Hygro	GenTarget	1x107 IFU/ml x 200ul	EUR 484.8
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Hygromycin antibiotic marker.

EF1a-TetR (Hygro) Lentiviral particles
LVP459-Hygro	GenTarget	1x107 IFU/ml x 200ul	EUR 484.8
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhaced EF1a promoter, with Hygromycin antibiotic marker.

CMV-TetR (Hygro) Lentiviral particles  in PBS
LVP017-Hygro-PBS	GenTarget	5x107 IFU/ml x 200ul	EUR 608.4
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Hygromycin antibiotic marker. Particles pellet was re-suspended in PBS solution.

EF1a-TetR (Hygro) Lentiviral particles  in PBS
LVP459-Hygro-PBS	GenTarget	5x107 IFU/ml x 200ul	EUR 608.4
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under Enahnced EF1a promoter, with Hygromycin antibiotic marker. Particles pellet was re-suspended in PBS solution.

AAV-1 Helper Free Bicistronic Expression System (Hygro)
VPK-417-SER1	Cell Biolabs	1 kit	EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-2 Helper Free Bicistronic Expression System (Hygro)
VPK-417-SER2	Cell Biolabs	1 kit	EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-3 Helper Free Bicistronic Expression System (Hygro)
VPK-417-SER3	Cell Biolabs	1 kit	EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-4 Helper Free Bicistronic Expression System (Hygro)
VPK-417-SER4	Cell Biolabs	1 kit	EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-5 Helper Free Bicistronic Expression System (Hygro)
VPK-417-SER5	Cell Biolabs	1 kit	EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-6 Helper Free Bicistronic Expression System (Hygro)
VPK-417-SER6	Cell Biolabs	1 kit	EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-DJ Helper Free Bicistronic Expression System (Hygro)
VPK-417-DJ	Cell Biolabs	1 kit	EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

AAV-DJ/8 Helper Free Bicistronic Expression System (Hygro)
VPK-417-DJ-8	Cell Biolabs	1 kit	EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

ViraSafe Lentiviral Expression System (Hygro), Ecotropic
VPK-214-ECO	Cell Biolabs	1 kit	EUR 1395.6
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Expression System (Hygro), Pantropic
VPK-214-PAN	Cell Biolabs	1 kit	EUR 1395.6
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

ViraSafe Lentiviral Bicistronic Expression System (Hygro), Ecotropic
VPK-217-ECO	Cell Biolabs	1 kit	EUR 1395.6
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

ViraSafe Lentiviral Bicistronic Expression System (Hygro), Pantropic
VPK-217-PAN	Cell Biolabs	1 kit	EUR 1395.6
Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)
1-CSB-YP321441SOO	Cusabio	EUR 814.80 EUR 402.00 EUR 2606.40 EUR 1261.20 EUR 1730.40 EUR 522.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase(bar) expressed in Yeast

Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)
1-CSB-EP321441SOO	Cusabio	EUR 733.20 EUR 370.80 EUR 2192.40 EUR 1126.80 EUR 1461.60 EUR 476.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase(bar) expressed in E.coli

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar
CM65-10ug	Novoprotein	10ug	EUR 187.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar
CM65-1mg	Novoprotein	1mg	EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar
CM65-500ug	Novoprotein	500ug	EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/Bar
CM65-50ug	Novoprotein	50ug	EUR 442.8
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)
RPC20112-100ug	Biomatik Corporation	100ug	EUR 721.6

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)
RPC20112-1mg	Biomatik Corporation	1mg	EUR 2884.7

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase (bar)
RPC20112-20ug	Biomatik Corporation	20ug	EUR 448.1

Recombinant Streptomyces hygroscopicus Phosphinothricin N-acetyltransferase/bar Protein, No Tag
E40PO147	EnoGene	20ug	EUR 495

We identify PIK3CA, RHOA, MAPK3, PAFAY1B1, CTNNB1, MYC, PRKCA, ERBB2, and CDC42 as a key gene on the MIR-375 network which is set and estimates the possible function of the MIR-375 in the heart muscle, which consists mainly in regulations on signaling paths that are dependent on on RHO-GTPases. We apply our algorithm to predict Mirna’s function into the python module, which is available at GitHub.