Background: Metastatic disease caused by prostate cancer (PCa) is the leading cause of PCa-related death. RNA long non-protein-coding may have a significant cellular functions. Plasmasitoma variant translocation 1 (PVT1), long non-coding RNA genes encoded by human PVT1, is an oncogene, which can set up multiple tumor-associated genes. In PCa, function and PVT1 mechanism is not clear. NOP2 being pursued as a prognostic marker for cancer aggressiveness, which promotes the growth of mouse fibroblast and tumor formation. Basically, nothing is known about the specific interaction between PVT1 and NOP2.

Methods: 190 pairs of PCa tissue and adjacent normal tissue were collected and RNA sequencing was used to identify differential lncRNAs. Real-time quantitative real-time PCR (RT-qPCR) confirmed these results and the relationship of gene regulation. PVT1 lentiviral vectors are used to alter and genes to analyze their effects on the development of PCa. Transwell migration and invasion assays were performed to test the ability of metastasis. Biofunction of PVT1 and NOP2 confirmed in vitro and in vivo.

Results: In this study, we report that long noncoding RNA-PVT1 is upregulated in PCa tissue promoted metastasis and PCa cell migration in vitro and their metastasis in vivo. High levels were significantly downregulated PVT1 tumor suppressor microRNAs (miRNAs), such as miR-15b-5p, miR-27a-3p, miR-143-3p, and miR-627-5p, levels in metastatic tissues are low compared with those in non-metastatic tissues. In vitro and in vivo, PVT1 promote PCa metastasis by targeting miRNAs. In addition, expression levels associated with the expression of positive PVT1 NOP2, cancer metastasis-associated protein. We show that promoted the invasion and migration NOP2 PCa. For certain mechanisms, correlation analysis showed that PVT1 promoted metastasis to up-regulate NOP2.

Conclusion: Overall, our results indicate that PVT1 acts as an inducer of PCa metastasis by targeting miRNAs, thus increasing NOP2. This axis may have diagnostic and therapeutic potential for advanced PCa.

Screening of potential microRNAs associated with pancreatic cancer: Data mining based on RNA sequencing and microarray

Pancreatic cancer is a malignant tumor of the digestive tract, rendering it difficult to make an accurate diagnosis. 5-year survival rate for pancreatic cancer is <1%, and surgical resection is rarely proven to be effective. Therefore, the identification of more effective methods for early detection of pancreatic cancer is an urgent need. This study aims to explore key genes and microRNAs (miRNAs) associated with the pathogenesis of pancreatic cancer. public databases were searched, and the integrated data from The Cancer Genome Atlas and Gene Expression Omnibus database, which leads to the identification of 23 miRNAs that are expressed differently (DE-miRNA).

Four of DE-regulated miRNAs (miR-892b-HSA, HSA-miR-194-2, miR-200a-HSA and HSA-miR-194-1), while the DE-19 downregulated miRNA (hsa- Mirza 424, HSA-mir-191, mir-484-HSA, HSA-miR-142, miR-15b-HSA, HSA-miR-450A-1, HSA-mir-423, mir-126-HSA, hsa- Mirza 505, HSA-miR-16-1, HSA-miR-342, miR-130A-HSA, HSA-miR-3613, HSA-miR-450A-2, HSA-miR-26b, miR-451-HSA, HSA- miR-19b-2, HSA-miR-106A and HSA-miR-503) were identified using a cut-off criteria of P <0.05 and | log 2FC |> 1.0.

Mouse C57 Mammary Gland, E12 cDNA
MD-414-12-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E13 cDNA
MD-414-13	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E13 cDNA
MD-414-13-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E14 cDNA
MD-414-14	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E14 cDNA
MD-414-14-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E15 cDNA
MD-414-15	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E15 cDNA
MD-414-15-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E16 cDNA
MD-414-16	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E16 cDNA
MD-414-16-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E17 cDNA
MD-414-17	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E17 cDNA
MD-414-17-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, E18 cDNA
MD-414-18	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, E18 cDNA
MD-414-18-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland Western Blot
MW-414	Zyagen	1 Blot	EUR 668

Mouse C57 Mammary Gland Western Blot
MW-414-C57	Zyagen	1 Blot	EUR 789

Mouse CD1 Mammary Gland, E11 Total RNA
MR-414-11	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E11 Total RNA
MR-414-11-C57	Zyagen	0.025mg	EUR 180

Mouse CD1 Mammary Gland, E12 Total RNA
MR-414-12	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E12 Total RNA
MR-414-12-C57	Zyagen	0.025mg	EUR 180

Mouse CD1 Mammary Gland, E13 Total RNA
MR-414-13	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E13 Total RNA
MR-414-13-C57	Zyagen	0.025mg	EUR 180

Mouse CD1 Mammary Gland, E14 Total RNA
MR-414-14	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E14 Total RNA
MR-414-14-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, E15 Total RNA
MR-414-15	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E15 Total RNA
MR-414-15-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, E16 Total RNA
MR-414-16	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E16 Total RNA
MR-414-16-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, E17 Total RNA
MR-414-17	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E17 Total RNA
MR-414-17-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, E18 Total RNA
MR-414-18	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, E18 Total RNA
MR-414-18-C57	Zyagen	0.05mg	EUR 180

Mouse Mammary Gland Developmental Western Blot
MW-414-D	Zyagen	1 Blot	EUR 910

Mouse CD1 Mammary Gland, E11 Total Protein
MT-414-11	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E11 Total Protein
MT-414-11-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E12 Total Protein
MT-414-12	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E12 Total Protein
MT-414-12-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E13 Total Protein
MT-414-13	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E13 Total Protein
MT-414-13-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E14 Total Protein
MT-414-14	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E14 Total Protein
MT-414-14-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E15 Total Protein
MT-414-15	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E15 Total Protein
MT-414-15-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E16 Total Protein
MT-414-16	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E16 Total Protein
MT-414-16-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E17 Total Protein
MT-414-17	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E17 Total Protein
MT-414-17-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, E18 Total Protein
MT-414-18	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, E18 Total Protein
MT-414-18-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, non pregnant cDNA
MD-414	Zyagen	30 reactions	EUR 243

Mouse CD1 Mammary Gland, non pregnant cDNA
MD-414-1	Zyagen	30 reactions	EUR 243

Mouse BLC Mammary Gland, non pregnant cDNA
MD-414-BLC	Zyagen	30 reactions	EUR 280

Mouse C57 Mammary Gland, non pregnant cDNA
MD-414-C57	Zyagen	30 reactions	EUR 280

Mouse C57 Mammary Gland, non pregnant cDNA
MD-414-C57-1	Zyagen	30 reactions	EUR 280

Mouse C57 Mammary Gland Frozen Sections, E11
MF-414-11-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E12
MF-414-12	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, E12
MF-414-12-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E11
MF-414-11	Zyagen	10 slides	EUR 253

Mouse CD1 Mammary Gland Frozen Sections, E13
MF-414-13	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, E13
MF-414-13-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E14
MF-414-14	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, E14
MF-414-14-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E15
MF-414-15	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, E15
MF-414-15-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E16
MF-414-16	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, E16
MF-414-16-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E17
MF-414-17	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, E17
MF-414-17-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, E18
MF-414-18	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, E18
MF-414-18-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E11
MP-414-11	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E11
MP-414-11-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E12
MP-414-12	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E12
MP-414-12-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E13
MP-414-13	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E13
MP-414-13-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E14
MP-414-14	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E14
MP-414-14-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E15
MP-414-15	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E15
MP-414-15-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E16
MP-414-16	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E16
MP-414-16-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E17
MP-414-17	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E17
MP-414-17-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, E18
MP-414-18	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, E18
MP-414-18-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Premade Northern Blot
MN-414	Zyagen	1 Blot	EUR 668

Mouse C57 Mammary Gland Premade Northern Blot
MN-414-C57	Zyagen	1 Blot	EUR 903

Mouse CD1 Mammary Gland Frozen Sections, Virgin
MF-414-V	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, Virgin
MF-414-V-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Virgin
MP-414-V	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Virgin
MP-414-V-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland, non pregnant Total RNA
MR-414	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, non pregnant Total RNA
MR-414-C57	Zyagen	0.025mg	EUR 180

Rabbit Mammary Gland cDNA
TD-414	Zyagen	30 reactions	EUR 243

Mouse Balbc Mammary Gland, non pregnant Total RNA
MR-414-BLC	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, non pregnant Total Protein
MT-414	Zyagen	0.5mg	EUR 153

Mouse BLC Mammary Gland, non pregnant Total Protein
MT-414-BLC	Zyagen	0.5mg	EUR 180

Mouse C57 Mammary Gland, non pregnant Total Protein
MT-414-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, Day 1 of Lactation cDNA
MD-414-L1	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, Day 1 of Lactation cDNA
MD-414-L1-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, Day 3 of Lactation cDNA
MD-414-L3	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, Day 3 of Lactation cDNA
MD-414-L3-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, Day 7 of Lactation cDNA
MD-414-L7	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, Day 7 of Lactation cDNA
MD-414-L7-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland Frozen Sections, Lactation D7
MF-414-L7	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D7
MF-414-L7-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D1
MF-414-L1	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D1
MF-414-L1-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Lactation D3
MF-414-L3	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, Lactation D3
MF-414-L3-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland, Day 1 of Involution cDNA
MD-414-N1	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, Day 1 of Involution cDNA
MD-414-N1-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, Day 7 of Involution cDNA
MD-414-N7	Zyagen	30 reactions	EUR 243

Mouse C57 Mammary Gland, Day 7 of Involution cDNA
MD-414-N7-C57	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland Frozen Sections, Involution D1
MF-414-N1	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, Involution D1
MF-414-N1-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Involution D7
MF-414-N7	Zyagen	10 slides	EUR 253

Mouse C57 Mammary Gland Frozen Sections, Involution D7
MF-414-N7-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D1
MP-414-L1	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D1
MP-414-L1-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D3
MP-414-L3	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D3
MP-414-L3-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Lactation D7
MP-414-L7	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Lactation D7
MP-414-L7-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Frozen Sections, Non-Pregnant
MF-414	Zyagen	10 slides	EUR 266

Mouse BLC Mammary Gland Frozen Sections, Non-Pregnant
MF-414-BLC	Zyagen	10 slides	EUR 291

Mouse C57 Mammary Gland Frozen Sections, Non-Pregnant
MF-414-C57	Zyagen	10 slides	EUR 277

Rat Mammary Gland, E12 cDNA
RD-414-12	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E13 cDNA
RD-414-13	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E14 cDNA
RD-414-14	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E15 cDNA
RD-414-15	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E16 cDNA
RD-414-16	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E17 cDNA
RD-414-17	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E18 cDNA
RD-414-18	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E19 cDNA
RD-414-19	Zyagen	30 reactions	EUR 243

Rat Mammary Gland, E20 cDNA
RD-414-20	Zyagen	30 reactions	EUR 243

Mouse CD1 Mammary Gland Paraffin Sections, Involution D1
MP-414-N1	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Involution D1
MP-414-N1-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Involution D7
MP-414-N7	Zyagen	10 slides	EUR 266

Mouse C57 Mammary Gland Paraffin Sections, Involution D7
MP-414-N7-C57	Zyagen	10 slides	EUR 291

Mouse CD1 Mammary Gland Paraffin Sections, Non-Pregnant
MP-414	Zyagen	10 slides	EUR 266

Mouse BLC Mammary Gland Paraffin Sections, Non-Pregnant
MP-414-BLC	Zyagen	10 slides	EUR 291

Mouse C57 Mammary Gland Paraffin Sections, Non-Pregnant
MP-414-C57	Zyagen	10 slides	EUR 291

Rat Mammary Gland Western Blot
RW-414	Zyagen	1 Blot	EUR 668

Rabbit Mammary Gland Total RNA
TR-414	Zyagen	0.05mg	EUR 160

Mouse CD1 Mammary Gland, Day 1 of Lactation Total RNA
MR-414-L1	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, Day 1 of Lactation Total RNA
MR-414-L1-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total RNA
MR-414-L3	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, Day 3 of Lactation Total RNA
MR-414-L3-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, Day 7 of Lactation Total RNA
MR-414-L7	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, Day 7 of Lactation Total RNA
MR-414-L7-C57	Zyagen	0.05mg	EUR 180

Mouse C57 Mammary Gland, non pregnant cDNA-Random Primer
MD-414-C57-RH	Zyagen	30 reactions	EUR 280

Mouse CD1 Mammary Gland, non pregnant cDNA-Random Primer
MD-414-HR	Zyagen	30 reactions	EUR 243

Mouse CD1 Mammary Gland, Day 1 of involution Total RNA
MR-414-N1	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, Day 1 of involution Total RNA
MR-414-N1-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, Day 7 of involution Total RNA
MR-414-N7	Zyagen	0.05mg	EUR 160

Mouse C57 Mammary Gland, Day 7 of involution Total RNA
MR-414-N7-C57	Zyagen	0.05mg	EUR 180

Mouse CD1 Mammary Gland, Day 1 of Lactation Total Protein
MT-414-L1	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, Day 1 of Lactation Total Protein
MT-414-L1-C57	Zyagen	0.5mg	EUR 180

Mouse CD1 Mammary Gland, Day 3 of Lactation Total Protein
MT-414-L3	Zyagen	0.5mg	EUR 153

Mouse C57 Mammary Gland, Day 3 of Lactation Total Protein
MT-414-L3-C57	Zyagen	0.5mg	EUR 180

Hsa-miR-3613-5p identified as prognostic DE-miRNA. Functional enrichment analysis showed that the target gene HSA-miR-3613-5p may be related to p53 signaling pathway.