Objective: It is currently available markers for the early detection of diabetic nephropathy (DN), the leading cause of end stage renal disease, has several limitations. There is ample evidence from previous studies on the role of several circulating microRNAs (miRNAs) in the early development of DN. This study aims to describe the expression of miRNA-377, miRNA-93, miRNA-25, miRNA-216A, and miRNA-21 in a sample of type 1 diabetic children and adolescents to explore their relationship with DN and several indices of renal injury. Patients and methods: Seventy type 1 diabetic patients with diabetes duration of 5 years or more, recruited from Children’s Hospital, Faculty of Medicine, Cairo University. Quantitative real-time reverse-transcription PCR (qRT-PCR) was used to measure the expression of the above mentioned miRNAs in serum and to assess its relationship with the DN, and the risk factors studied.
Results: There were significantly higher percentage of up-regulation of miRNA-377 and miRNA-93 (P = 0.03, 0.02, respectively) in addition to significant down-regulation of miRNA-25 (P = 0.01) in patients with DN than in patients without DN. In patients with DN, the expression of miR-216A were significantly negatively correlated with creatinine (r = -0.4, P = 0.04) and positively correlated with eGFR using creatinine (r = 0.5, P = 0.03). In the same group, the expression of miR-21 was positively correlated with urinary cystatin C (r = 0.6, P = 0.01) and negatively correlated with e-GFR using cystatin C (r = -0.6, P = 0, 01).
MiRNA-93 was associated with an increased risk (odds ratio = 15, 95% CI = 12.03 to 24.63, P = 0.01), whereas miRNA-25 was associated with a decreased risk of albuminuria (odds ratio = 0.15, 95 % CI = 0.08- 0.55; P = 0.03)
Conclusion: miRNA-377, miRNA-93, miRNA-216A, and miRNA-21 may be involved in the pathogenesis of DN, whereas miRNA-25 may have a reno-protective role. More studies are needed to document the value of these miRNAs as diagnostic biomarkers and therapeutic targets in DN.
MicroRNAs and Risk Factors for Diabetic Nephropathy in Egyptian Children and Adolescents with Type 1 Diabetes
MicroRNA Profiling in Paired Left and Right Eye, Lung, and Normal Mice Testes
Extensive physiological and pathophysiological differences exist in paired organ systems. However, the molecular basis for this difference is unknown. We previously reported that miRNAs are differentially expressed (DEMs) on the left and right kidneys of normal mice.
Here, we identified Dems on the left and right eyes, lungs, and testes of normal mice via RNA sequencing. As a result, we identified 26 Dems in the eye, with 23 higher and 3 lower in the left eye compared to the right eye; 21 Dems in the lungs, with 15 higher and lower 6 in left lung compared to right lung; and 54 Dems in the testicles, with 6 higher and 48 lower in the left testicle than the right testis. ten microRNAs (miRNAs) are subsequently examined by quantitative PCR test, and seven of them were confirmed. In addition, correlation analysis was conducted between paired organ miRNA expression and miRNA expression of a variety of body fluids.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47A(CT47A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen 47B(CT47B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 1(CTAG1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Cancer/testis antigen 2(CTAG2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Bromodomain testis specific protein(BRDT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Bromodomain testis specific protein(BRDT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Bromodomain testis specific protein(BRDT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Bromodomain testis specific protein(BRDT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey Testis expressed sequence 10 protein(TEX10) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Human testis tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human testis tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated testis tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated testis tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Fetal and adult testis expressed transcript protein(FATE1) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Fetal and adult testis expressed transcript protein(FATE1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A1(CT45A1) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A1(CT45A1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A2(CT45A2) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A2(CT45A2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A3(CT45A3) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A3(CT45A3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A5(CT45A5) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A5(CT45A5) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This cell lysate is prepared from rat testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: This cell lysate is prepared from mouse testis tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: Human testis tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human testis tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the testis tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The testis tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Human testis tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human testis tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated testis tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated testis tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Cancer/testis antigen family 45 member A4/A6(CT45A4) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Testis Tumor Tissue Array - 16 cases of testis tumors and 8 cases of non-tumor testis tissues.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: TES Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 441amino acids (1-421 a.a.) and having a molecular mass of 50.1 kDa. The TES protein is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Finally, we explore the function and tissue Dems and conducted biological processes and target gene pathway enrichment analysis for Dems, providing insights into the physiological and pathophysiological differences between the two entities are paired organs. The article “Knockdown of microRNA-181a inhibits osteosarcoma cell growth and invasion through pyroptosis NLRP3-dependent triggering, by B.-G. Tian, Z. Hua, Z.-J. Wang, J. Li, published in Eur Rev Med Pharmacol Sci 2020 have been drawn from the author. Publisher apologizes for any inconvenience this may cause.
Douglas
