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miRNAs may play a major role in the control of gene expression in key pathobiological processes in Chagas disease cardiomyopathy
Chronic Chagas Disease Cardiomyopathy (CCC), a very aggressive cardiomyopathy of inflammation caused by a lifetime infection with the Protozoa Trypanosoma Cruzi, is the main cause of cardiomyopathy in Latin America. Although chronic myocarditis can play the main pathogenetic role, a little known about the molecular mechanism responsible for its severity. The purpose of this study was to study micrornas genes and expressions in their networks and connections in connection with the pathobiological process. To do this, we are integrated for the first time a global microrna expression and MRNA from the CCC patient’s myocardial network that uses pathways and network analysis. We observe enrichment in biological processes and lines related to immune response and metabolism. IFNγ, TNF and NFKB are top upstream regulators.
The intersection between Micrornas disclosed differently and the MRNAS target expressed differently shows enrichment in biological processes such as inflammation, inflammation, genes, fibrosis, hypertrophy, and mitochondrofil / oxidative antioxidant response. Micrornas also plays a role in gene expression regulations involved in the process related to cardiomyopathy related to fibrosis, hypertrophy, myocarditis and arrhythmias. Significantly, the number of discrete micrillah which is expressed differently targets the high number of MRNAS expressed differently (> 20) in several processes. Our results show that MInnas orkestrate expression is a lot of genes in the main pathophysiological process in CCC’s heart tissue. It may have pads on pathogenesis, biomarkers and therapy.
Impact of zinc oxide nanoparticles in cytotoxicity, genotoxicity, and Mirna expression in Barley (Hordeum Vulgare L.) Seeds
Nanoparticles Zinc oxide is one of the most commonly engineered nanomaterials and must enter the environment because of the large amount produced and the application is widespread. Understanding the impact of nanoparticles in plant growth and development is very important for assessing possible environmental risks to food security and human health, because plants are a basic component of living from ecosystems and the most important sources in the human food chain.
The purpose of this study was to examine the impact of various concentrations of zinc oxide nanoparticles in the seed germination of Barley Hordeum L. seeds, seed morphology, root cell viability, stress level, genotoxicity, and MIRNAS’s expression. The results showed that zinc oxide nanoparticles increased barley seed germination, climbing / root lengthening, and H2O2 stress levels and reduced the root cell viability and stability of the Genome and MIRNAS templates and downregated in wheat seeds.
Mirna’s dynamics mediate legume symbiosis regulations
Fixation of symbiotic nitrogen in legal nodules is important in land with low nitrogen availability. Symbiosis initiation and sustainability requires cellular reprogramming that involves inhibition of minna or activation of certain nodulation genes. High sementing from a small RNA library has identified the MIRNAS and their target, which is a major player in the post-transcription gene regulation (PTGS) from various stages of legum-rhizobia symbiosis ranging from bacterial and organogenesis colonization to symbiotic nitrogen fixation. Here we present an overview of information obtained from the Mirna Library from the sorted nodulation network to date.
MIRNAS’s functional analysis has revealed the role in phytohormone homeostasis and spatio-temporal regulations, as well as MIRNAS mobility and its function in root signaling that affects various functions, including bacterial entries, meristem divisions, nitrogen and aging fixation. Furthermore, the small RNA fragment from Rhizobial from pressing MRNA complementary plants. We also consider the role of MIRNAS in determining decisive or uncertain nodules. Taken together, this overview confirms that MIRNAS is the main regulator of the symbiosis of Legum-Rhizobia. This article is protected by copyright. All copyrights.
Identification and validation of the MIRNAS key and microrna-mrna settings networks related to ulcerative colitis
Olcerativa (UC) colitis is a chronic intestinal inflammatory disease, not specific, which involves various genes and paths in their pathogenesis. The current study reveals the main MIRNAS and network regulation of potential target genes as a model to predict the UC molecular mechanism. This can provide new insights to uncover UC pathogenesis. Differential declares MIRNAS (Demis) and MRNA (DEGS] which are expressed differently between UC patients and normal control of discretion using the omnibus database of gene expression. The target gene to be predicted to use the MIRDB, Mirwalk, Starbase, Tarbase and TargetScan data basis, and the Mirna network -MRNA was formed using the DEG which was changed in the opposition to Demis.
10x PBS Ready Mixed Powder |
BA01701 |
Bioatlas |
1L |
EUR 81.6 |
Description: High purity buffer for various PCR applications. |
10X PBS MaxTag Histo (OKRA00043) |
OKRA00043 |
Aviva Systems Biology |
100 ml |
EUR 168 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
10X PBS Buffer , pH 7.4, Refill Pack |
P2100-204 |
GenDepot |
3.5L |
EUR 165.6 |
10X PBS Buffer , pH 7.4, Refill Pack |
P2100-208 |
GenDepot |
8L |
EUR 262.8 |
Phosphate Buffered Saline (PBS) 10x pH 7.4, 1000 ml |
12-9423-5 |
Medicago |
1000 ml |
EUR 118.8 |
T-Pro Washing buffer in PBS and Tween-20 (10X) |
JB09-I003 |
T-Pro Biotechnology |
500ml/BT |
EUR 172.8 |
T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing |
JB09-I001 |
T-Pro Biotechnology |
500ml/BT |
EUR 162 |
10x PBST Solution (10X Phosphate Buffered Saline, 0.5% Tween-20), PH 7.4 |
UPD8119 |
Bio Basic |
500ml |
EUR 80.88 |
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PBST buffer 10x Strength Solution |
PW004 |
Bio Basic |
500ml |
EUR 80.88 |
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PBS |
abx098132-500ml |
Abbexa |
500 ml |
EUR 210 |
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PBS Buffer |
RM00012 |
Abclonal |
2L |
EUR 122.4 |
PBS Isotonic Buffer, 1-Liter; 20X Concentrate |
NB303 |
Innovex |
1 Liter |
EUR 289 |
Description: PBS Isotonic Buffer, 1-Liter; 20X Concentrate |
Trypsin, 2.5%(10X) |
CA019-010 |
GenDepot |
100ml |
EUR 96 |
10x Taq Buffer |
BA00151 |
Bioatlas |
1.8ml |
EUR 66 |
Description: High purity buffer for various PCR applications. |
NH4 Buffer, 10x |
BIO-37025 |
Bioline |
3 x 1.2ml |
Ask for price |
10X TAE Buffer |
T8048-101 |
GenDepot |
1L |
EUR 106.8 |
TBS Buffer, 10X |
T8057-100 |
GenDepot |
1L |
EUR 138 |
TBS Buffer, 10X |
T8057-105 |
GenDepot |
5x1L |
EUR 343.2 |
10X MOPS Buffer |
M1057-050 |
GenDepot |
500ml |
EUR 193.2 |
10X MOPS Buffer |
M1057-100 |
GenDepot |
2X500ml |
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Phosphate Buffered Saline (PBS, pH 7.4) concentrate (20X) |
80081 |
Alpha Diagnostics |
100 ml |
EUR 196.8 |
10x Proteinase K |
TG4039 |
TopoGen |
0.5ml |
EUR 160.8 |
10X Loading Buffer |
L0503-005 |
GenDepot |
5X1ml |
EUR 92.4 |
10X Loading Buffer |
L0504-005 |
GenDepot |
5X1ml |
EUR 92.4 |
Blocking Buffer, 10X, 250ML |
X109-250ML |
Arbor Assays |
250ML |
EUR 360 |
Collagenase/Hyaluronidase (10X) |
CA094-002 |
GenDepot |
20ml |
EUR 408 |
TBST(10X), pH 7.4 |
T8056-100 |
GenDepot |
1L |
EUR 138 |
10x HAT Assay Buffer |
50095 |
BPS Bioscience |
20 ml |
EUR 40 |
Description: Buffer optimized for use with BPS Bioscience's histone acetyltransferase (HAT) assay kits. |
10x Taq Buffer (MgCl2) |
PCRB16 |
Bio Basic |
4x1.5ml, 6ml |
EUR 70.44 |
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PBS Tablets (1000 ml) |
2129-100 |
Biovision |
each |
EUR 764.4 |
PBS Tablets (100 ml) |
2865-100 |
Biovision |
each |
EUR 196.8 |
PBS Tablets (1000 ml) |
2129-10 |
Biovision |
each |
EUR 196.8 |
10X KRB-IBMX Buffer |
122937 |
Mediomics |
1 Vial |
EUR 92.4 |
10x PARP Assay Buffer |
80602 |
BPS Bioscience |
1 ml |
EUR 45 |
Description: The 10x PARP Assay Buffer is optimized for use with BPS_x000D_Bioscience's poly(ADP) ribose polymerase (PARP) assay kits. |
Trypsin-EDTA(10X), 0.5% |
CA015-010 |
GenDepot |
100ml |
EUR 105.6 |
Trypsin-EDTA(10X), 0.5% |
CA015-100 |
GenDepot |
10x100ml |
EUR 343.2 |
Trypsin-EDTA(10X), 0.5% |
CA015-200 |
GenDepot |
20x100ml |
EUR 578.4 |
Trypsin-EDTA(10X), 0.05% |
CA022-010 |
GenDepot |
100ml |
EUR 100.8 |
RBC Lysis Solution 10X |
abx290033-50ml |
Abbexa |
50 ml |
EUR 276 |
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RBC Lysis Solution 10X |
RBC10X-50ML |
ImmunoStep |
50 ML |
EUR 130.2 |
10x Gel Loading Buffer |
TG4038 |
TopoGen |
1ml |
EUR 187.2 |
10X TAE buffer solution |
A00239 |
Bio Basic |
500ml |
EUR 67.63 |
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10x TAE Buffer Solution |
BA01801 |
Bioatlas |
6x100ml |
EUR 116.4 |
Description: High purity buffer for various PCR applications. |
10x TBE Buffer Solution |
BA01804 |
Bioatlas |
6x100ml |
EUR 116.4 |
Description: High purity buffer for various PCR applications. |
Blocking Buffer, 10X, 25ML |
X109-25ML |
Arbor Assays |
25ML |
EUR 59 |
PBS Wash Buffer Pack |
AR1155 |
BosterBio |
500mL/pack |
EUR 78 |
10x Cell Isolation Buffer |
78563 |
BPS Bioscience |
5 ml |
EUR 25 |
Description: This buffer is intended for use with mammalian cells during magnetic cell isolation or single cell processing. This buffer is optimized and formulated to reduce nonspecific binding and cell clumping |
Binding/Coating Buffer (10X) |
85R-124 |
Fitzgerald |
250 ml |
EUR 259.2 |
Description: ELISA buffer for optimal coating and binding of antibodies and antigens |
Alkaline Phosphate Buffer, 10x |
K2191050-6 |
Biochain |
50 ml |
EUR 164.4 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
ToxOut? Endotoxin Free PBS |
7943-50 |
Biovision |
each |
EUR 222 |
10x PTP1B Colorimetric Substrate |
79693 |
BPS Bioscience |
5 ml |
EUR 120 |
Description: 10x PTP1B Colorimetric Substrate for use with BPS Bioscience's PTP1B Colorimetric Assay Kit (#30019). |
EZBlock? (PBS) Blocking Buffer |
2128-1000 |
Biovision |
each |
EUR 274.8 |
EZBlock? (PBS) Blocking Buffer |
2128-200 |
Biovision |
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EUR 164.4 |
SEAP (CAG, Bsd) in PBS |
LVP1203-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under CAG promoter, containing Blasticidin selection. |
SEAP (CAG, Neo) in PBS |
LVP1204-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP unde CAG promoter, containing Neomycin selection. |
1X PBS Buffer , pH 7.4 |
P2101-050 |
GenDepot |
500ml |
EUR 79.2 |
1X PBS Buffer , pH 7.4 |
P2101-100 |
GenDepot |
1L |
EUR 105.6 |
SEAP (EF1a, Bsd) in PBS |
LVP1194-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Blasticidin selection. |
SEAP (EF1a, Neo) in PBS |
LVP1195-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP unde EF1a promoter, containing Neomycin selection. |
SEAP (EF1a, Zeo) in PBS |
LVP1201-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Zeomycin selection. |
SEAP (CAG, Puro) in PBS |
LVP1202-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under CAG promoter, containing puromycin selection. |
SEAP (EF1a, GFP) in PBS |
LVP1217-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under EF1a promoter with GFP fluorescent marker. |
SEAP (EF1a, RFP) in PBS |
LVP1218-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under EF1a promoter with RFP fluorescent marker. |
SEAP (Ubc, Puro) in PBS |
LVP1219-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under Ubc promoter, containing puromycin selection. |
Phosphate Buffered Saline (PBS) |
2113-500 |
Biovision |
each |
EUR 164.4 |
SEAP (EF1a, Puro) in PBS |
LVP1193-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing puromycin selection. |
SEAP (mPGK, Puro) in PBS |
LVP1220-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under mPGK promoter, containing puromycin selection. |
SEAP (ActB, Puro) in PBS |
LVP1221-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under ActB promoter, containing puromycin selection. |
10x Taq Buffer (NH4)2SO4 |
PCRB55 |
Bio Basic |
4x1.5ml, 6ml |
EUR 70.44 |
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SEAP (TetCMV, Bsd) in PBS |
LVP1185-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Blasticidin selection. |
SEAP (TetCMV, Neo) in PBS |
LVP1186-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Neomycin selection. |
SEAP (TetCMV, Zeo) in PBS |
LVP1192-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter, containing Zeomycin selection. |
SEAP (EF1a, Hygro) in PBS |
LVP1200-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under EF1a promoter, containing Hygromycin selection. |
SEAP (TetCMV, GFP) in PBS |
LVP1215-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter with GFP fluorescent marker. |
SEAP (TetCMV, RFP) in PBS |
LVP1216-PBS |
GenTarget |
1x107 IFU/ml x 200ul |
EUR 852 |
Description: Concentrated Lentivirus express SEAP under optional inducible TetCMV promoter with RFP fluorescent marker. |
10X Citrate Buffer *pH 6.0* |
10000 |
AAT Bioquest |
100 mL |
EUR 97 |
We verify the main expression of Demis in the UC model of rodents. The Mirna-MRNA network contains 31 Demis and 199 degrees, which show enrichment in inflammation of the intestinal Hub. In addition, we identify six Demis and genes from initial validation analysis in the network model. In the pathophysiological process of UC, various genes and proteins display differences of expressions and complex interactions with each other. This finding provides new insight into the potential key mechanism Related to UC development.