Inhibiting roles of long non-coding RNA OIP5-AS1 in rheumatoid arthritis progression through the microRNA-448/PON1/TLR3/NF-κB axis
What is the main question of this study? This study aims to explore the OIP5-AS1 lncRNA function in rheumatoid arthritis inflammation and development and molecular mechanisms. What are the main findings and importance? LncRNA relieve rheumatoid arthritis OIP5-AS1 through the network Cerna developments involving miR-448 / PON1 axis and through inactivation of TLR3 / NF-kB signaling pathway. This research may offer new ideas for molecular-based control for rheumatoid arthritis Abstract: Rheumatoid arthritis (RA) is an autoimmune disorder with dysregulation of long non-coding RNA (lncRNAs) may be involved.
This study aims to inquire into OIP5-AS1 lncRNA role in the development of RA. A mouse model with RA induced. Excess of OIP5-AS1 are introduced in a mouse model, and then change leg swelling, RA severity and inflammatory factors IL-1β, IL-10, IL-6 and TNF-α measured. Fibroblast like synoviocytes (FLS) from RA patients were collected for in vitro experiments. Gain- and loss of function OIP5-AS1, miR-448 and paraoxonase 1 (PON1) is conducted to explore their role in RA-FLS growth, apoptosis, and inflammation. A specific TLR3 agonist or antagonist PIC-specific NF-kB QNZ was administered in RA-FLS. As a result, overexpression OIP5-AS1 reduce symptoms and severity and levels of inflammatory factors in rats RA.
OIP5-AS1 can bind to miR-448 to enhance the expression of PON1. Further excess miR-448-AS1 OIP5 reverse effect, while overexpression of PON1 inhibits the growth of RA-FLS and inflammation. In addition, activation of TLR3 promoted the development of RA. To conclude, this study demonstrated that lncRNA OIP5-AS1 can reduce the progression of RA through the miR-448 / PON1 axis and through inactivation of TLR3 / NF-kB signaling pathway. This article is protected by copyright. All rights reserved.
Inhibiting roles of long non-coding RNA OIP5-AS1 in rheumatoid arthritis progression through the microRNA-448/PON1/TLR3/NF-κB axis
Perk-Mediated Suppression of microRNAs by Sildenafil Improves Mitochondrial Dysfunction in Heart Failure
Oxidative / nitrosative stress is a major trigger of cardiac dysfunction, which involves a folded protein response and mitochondrial dysfunction. Activation of the nitric oxide-cyclic guanosine monophosphate kinase G-protein signaling by sildenafil increases the heart mal-remodeling during heart failure-induced excess pressure. Transcriptome analysis conducted in heart failure with or without sildenafil treatment. Protein kinase R-like endoplasmic reticulum (ER) kinase (perk) downstream signaling pathways, EIF2 and NRF2, significantly altered.
Although EIF2 signal is suppressed, NRF2 signaling is upregulated, inhibits the maturation of miR-24-3p through EGFR mediated phosphorylation Ago2. To study the effects of sildenafil on this path, we produce specific heart perk KO mice. In these mice, sildenafil could not inhibit maturations, nuclear translocation NRF2 pressed, and advanced mitochondrial dysfunction. Overall, these results suggest that the perk-mediated suppression miRNAs by sildenafil is essential for maintaining homeostasis of mitochondria through oxidative stress response NRF2-mediated.
Lef-1 role in the development of ectodermal organs have been characterized using Lef-1 knockout murine models. We produce lef-1 conditional over-expression (Coel) mouse to determine the role of Lef-1 expression in epithelial structure at the final stage of development after the endogenous expression of the switch to the mesenchyme. Let-1 over expression (OE) in the oral epithelium creating new niche dental epithelial stem cells that significantly enhance the growth of the incisors.
ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Rat Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Porcine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELI
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Troponin C, slow skeletal and cardiac muscles in samples from serum, plasma, tissue homogenates and other biological fluids.
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Chicken Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1)
Description: Quantitative sandwich ELISA for measuring Mouse Troponin C, slow skeletal and cardiac muscles (TNNC1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Goat Troponin C, slow skeletal and cardiac muscles (TNNC6)
Description: Quantitative sandwich ELISA for measuring Goat Troponin C, slow skeletal and cardiac muscles (TNNC6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)
Description: This cell lysate is prepared from rat skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Bovine skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Monkey (Cynomolgus) skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skeletal muscle tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skeletal muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skeletal muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Skeletal Muscle tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Skeletal muscle Actinin Alpha in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: human skeletal muscle tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
Description: human skeletal muscle tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin, Alpha skeletal muscle(ACTA1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Muscle, skeletal, receptor tyrosine kinase ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Muscle, skeletal, receptor tyrosine kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Actin Alpha 1, Skeletal Muscle in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in tissue homogenates, cell lysates and other biological fluids.
Rat Ryanodine Receptor 1, Skeletal (RYR1) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Ryanodine Receptor 1, Skeletal (RYR1) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: A Mouse Monoclonal antibody against ? skeletal muscle actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC
Description: A Mouse Monoclonal antibody against ? skeletal muscle actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC
Description: A Rabbit Polyclonal antibody against ?-Skeletal Muscle Actin from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC
Description: This cell lysate is prepared from mouse skeletal muscle tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: human skeletal muscle tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human skeletal muscle tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the Skeletal Muscle tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The Skeletal Muscle tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, slow skeletal muscle (TNNI1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, slow skeletal muscle(TNNI1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, fast skeletal muscle (TNNI2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin I, fast skeletal muscle(TNNI2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, slow skeletal muscle (TNNT1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat Troponin T, slow skeletal muscle(TNNT1) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, slow skeletal muscle(TNNT1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, fast skeletal muscle (TNNT3) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat Troponin T, fast skeletal muscle(TNNT3) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Actin Alpha 1, Skeletal Muscle (ACTa1) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Actin Alpha 1, Skeletal Muscle (ACTa1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Tnni2(Troponin I, fast skeletal muscle) ELISA Kit
Description: A sandwich ELISA kit for detection of Ryanodine Receptor 1, Skeletal from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Troponin I, fast skeletal muscle(TNNI2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Troponin I, fast skeletal muscle(TNNI2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Troponin T, fast skeletal muscle(TNNT3) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Troponin T, fast skeletal muscle(TNNT3) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Tnni1/ Troponin I, slow skeletal muscle ELISA Kit
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Troponin T, fast skeletal muscle in samples from serum, plasma, tissue homogenates and other biological fluids.
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These data indicate that Lef-1 expression is switched off in the dental epithelium at an early stage to maintain the stem cell niche and set incisors growth. Bioinformatics analysis showed that the expression of miR-26b increases coincided with a decrease in Lef-1 expression in epithelial teeth.
